Sequence of the domestic duck (Anas platyrhynchos) growth hormone‐encoding gene and genetic variation in the promoter region
Sequence of the domestic duck (Anas platyrhynchos) growth hormone‐encoding gene and genetic variation in the promoter region
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DOI：
10.1111/j.1740-0929.2008.00513.x
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发表时间：
2008-04
影响因子：
2
通讯作者：
N. Kansaku;A. Soma;S. Furukawa;Gen Hiyama;Hisato Okabayashii;D. Guemene;U. Kuhnlein;D. Zadworny
中科院分区：
文献类型：
--
作者：
N. Kansaku;A. Soma;S. Furukawa;Gen Hiyama;Hisato Okabayashii;D. Guemene;U. Kuhnlein;D. Zadworny
用聚合酶链式反应(PCR)扩增鸭生长激素基因及其启动子区域。共克隆和测序了5.25kb的片段。鸭生长激素(GH)由5个外显子和4个内含子组成，在结构上与哺乳动物和鸡的生长激素基因相似。虽然鸭生长激素启动子的远端区域与鸡和火鸡的启动子没有相似性，但启动子的近端含有两个推测的Pit-1结合序列，并与鸡和火鸡的生长激素启动子具有相似性。在启动子区域的5个位置检测到遗传变异。本研究结果表明，鸭生长激素基因的表达可能通过增强子型顺式作用元件以与鸡生长激素相似的方式进行调控，鸭生长激素基因的遗传变异可能适用于分子标记辅助选择。
The duck growth hormone encoding gene and its promoter region were amplified by polymerase chain reaction (PCR). A total of 5.25 kb were cloned and sequenced. Duck growth hormone (GH) consists of five exons and four introns and is structurally similar to mammalian and chicken GH gene. Although the distal region of duck GH promoter showed no similarity to chicken and turkey promoters, the proximal region of the promoter contained two putative Pit-1 binding sequences, and showed similarity to chicken and turkey GH promoters. Genetic variation was detected at five positions of the promoter region. The results of this study indicate that the expression of duck GH is likely regulated in a similar manner to that of chicken GH via enhancer-type cis-acting elements and the presence of genetic variation in the duck GH gene may be applicable to marker-assisted selection.