The actin multigene family in Populus: organization, expression and phylogenetic analysis
The actin multigene family in Populus: organization, expression and phylogenetic analysis
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杨树肌动蛋白多基因家族：组织、表达和系统发育分析
DOI：
10.1007/s00438-010-0552-5
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发表时间：
2010-08-01
影响因子：
3.1
通讯作者：
Li, Bailian
中科院分区：
文献类型：
--
作者：
Zhang, Deqiang;Du, Qingzhang;Li, Bailian
尽管肌动蛋白在植物生长发育中具有重要意义，但对木本植物肌动蛋白基因家族的结构、表达和进化却知之甚少。在这项研究中，我们系统地研究了肌动蛋白基因家族的多样性，在杨树整合基因组组织，表达和胚胎发生数据。杨树基因组的全基因组分析表明，肌动蛋白是一个由8个成员组成的多基因家族，所有成员预测编码377个氨基酸的多肽，具有94.2%至100%的同源性。微阵列和实时PCR表达分析表明，PtrACT家族成员在不同组织中差异表达，表现出重叠和独特的表达模式。特别令人感兴趣的是，所有的PtrACT基因已被发现优先表达在茎韧皮部和木质部，表明白杨PtrACT参与木材的形成。基因结构和系统发育分析表明，PtrACT家族是由两个主要的亚群，共享一个古老的共同祖先。检测到极高的种内同义核苷酸多样性pi（syn）= 0.01205，并且pi（non-syn）/pi（syn）比率显著小于1;因此，PtACT 1似乎在杨树中进化，主要是在纯化选择下。我们证明，肌动蛋白基因家族在杨树分为两个不同的亚群，表明功能分歧。本文的研究结果将有助于进一步开展功能基因组学研究，以了解肌动蛋白基因在树木生长发育中的详细功能。
Despite the significance of actin in plant growth and development, little is known of the structure, expression and evolution of the actin gene family in woody plants. In this study, we systematically examined the diversification of the actin gene family in Populus by integrating genomic organization, expression, and phylogeny data. Genome-wide analysis of the Populus genome indicated that actin is a multigene family consisting of eight members, all predicted to encode 377-amino acid polypeptides that share high sequence homology ranging from 94.2 to 100% identity. Microarray and real-time PCR expression analysis showed that the PtrACT family members are differentially expressed in different tissues, exhibiting overlapping and unique expression patterns. Of particular interest, all PtrACT genes have been found to be preferentially expressed in the stem phloem and xylem, suggesting that poplar PtrACTs are involved in the wood formation. Gene structural and phylogenetic analyses revealed that the PtrACT family is composed of two main subgroups that share an ancient common ancestor. Extremely high intraspecies synonymous nucleotide diversity of pi(syn) = 0.01205 was detected, and the pi(non-syn)/pi(syn) ratio was significantly less than 1; therefore, the PtACT1 appears to be evolving in Populus, primarily under purifying selection. We demonstrated that the actin gene family in Populus is divided into two distinct subgroups, suggesting functional divergence. The results reported here will be useful in conducting future functional genomics studies to understand the detailed function of actin genes in tree growth and development.