SARS coronavirus E protein in phospholipid bilayers: An X-ray study
SARS coronavirus E protein in phospholipid bilayers: An X-ray study
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DOI：
10.1529/biophysj.105.072892
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Salditt, T
中科院分区：
文献类型：
--
作者：
Khattari, Z;Brotons, G;Salditt, T
我们研究了严重急性呼吸综合征E蛋白模型脂质膜的疏水结构域的X-射线反射率和X-射线散射。特别是，我们使用X射线反射率来研究脂质双层内的碘标记残基的位置。标签对蛋白质拓扑结构施加了空间限制。实验数据作为蛋白质/脂质比P/L和不同溶胀状态的函数，支持先前报道的严重急性呼吸综合征E蛋白的发夹构象。双层厚度和酰基链的顺序的变化作为P/L的函数，并讨论了在不同的结构模型。
We investigated the structure of the hydrophobic domain of the severe acute respiratory syndrome E protein in model lipid membranes by x-ray reflectivity and x-ray scattering. In particular, we used x-ray reflectivity to study the location of an iodine-labeled residue within the lipid bilayer. The label imposes spatial constraints on the protein topology. Experimental data taken as a function of protein/lipid ratioP/L and different swelling states support the hairpin conformation of severe acute respiratory syndrome E protein reported previously. Changes in the bilayer thickness and acyl-chain ordering are presented as a function of P/L, and discussed in view of different structural models.