Molecular cloning and expression analysis of a fish specific interferon regulatory factor, IRF11, in orange spotted grouper, Epinephelus coioides.
Molecular cloning and expression analysis of a fish specific interferon regulatory factor, IRF11, in orange spotted grouper, Epinephelus coioides.
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斜带石斑鱼鱼类特异性干扰素调节因子 IRF11 的分子克隆和表达分析。
DOI：
10.1016/j.fsi.2016.12.007
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Nie P
中科院分区：
文献类型：
--
作者：
Huang Wen Shu;Zhu Mei Hua;Chen Shan;Wang Zhi Xuan;Liang Ying;Huang Bei;Nie P
干扰素调节因子（Interferon regulatory factors，IRFs）是一类转录调节因子，在抗病毒防御、免疫应答、细胞生长调节和细胞凋亡等多种生物学过程中发挥重要作用。在以往的研究中，通过检索鱼类基因组数据库，发现了一个鱼类特异性IRF，命名为IRF 11。本研究从斜带石斑鱼（Epinealphanus coioides）中克隆了IRF 11的转录本EcIRF 11。EcIRF 11 cDNA序列长1573 bp，编码261个氨基酸，与硬骨鱼EcIRF 11的同源性较高。在硬骨鱼基因组的比较分析显示，IRF 11可能有一个古老的起源至少4.5亿年前，和位点窝藏IRF 11可能经历了染色体重排和/或倒位在进化过程中。表达分析表明，IRF 1和IRF 2也在IRF 1亚组（SG）中，作为IRF 11，在实验感染早期对病毒刺激poly I：C和细菌刺激副溶血性弧菌感染的鱼中表现出高表达水平，而EcIRF 11在检测的时间点除了在poly I：C刺激后6 h在肾脏中没有转录调节。综上所述，本研究中获得的结果表明，IRF 11可能与IRF 1和IRF 2起源于相同的祖先，但表现出不同的基础和诱导表达，这意味着其不同的功能，需要进一步表征。
Interferon regulatory factors (IRFs) are transcription mediators which play vital roles in multiple biological processes, such as antiviral defense, immune response, cell growth regulation and apoptosis. A fish specific IRF, termed IRF11, has been identified in previous study through searching fish genome databases. Herein, a transcript of IRF11, EcIRF11 was cloned from orange-spotted grouper,Epinephelus coioides. The EcIRF11 cDNA sequence has 1573 bp in length, encoding a putative protein of 261 amino acids, with a high degree of similarity found between EcIRF11 and its teleost counterparts. Comparative analyses in teleost genomes revealed that IRF11 may have an ancient origin at least 450 million years ago, and the locus harbouring IRF11 might have experienced chromosomal rearrangement and/or inversion during evolution. Expression analysis revealed that the other two members, IRF1 and IRF2 also in the IRF1 subgroup (SG) as IRF11, exhibited high expression levels in early experimental infection phase in response to viral stimulation of poly I:C and to bacterial stimulation ofVibrio parahaemolyticusinfections in the fish, while EcIRF11 is not transcriptionally modulated at the examined time points except in kidney at 6 h following poly I:C stimulation. Taken together, the results obtained in this study indicate that IRF11 might have been originated from the same ancestor as IRF1 and IRF2, but exhibits distinct basal and induced expression, implying its different function which needs further characterization.