USING KNOWN SUBSTRUCTURES IN PROTEIN MODEL-BUILDING AND CRYSTALLOGRAPHY
USING KNOWN SUBSTRUCTURES IN PROTEIN MODEL-BUILDING AND CRYSTALLOGRAPHY
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DOI：
10.1002/j.1460-2075.1986.tb04287.x
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发表时间：
1986-04-01
期刊：
影响因子：
11.4
通讯作者：
THIRUP, S
中科院分区：
文献类型：
--
作者：
JONES, TA;THIRUP, S
视黄醇结合蛋白可以从三种不相关蛋白质的少量大型亚结构中构建。已知的结构被视为一个知识库，从中提取信息，用于分子建模时，缺乏真正的原子分辨率。这包括解释电子密度图和模拟同源蛋白质。可以更准确、更快速地将模型构建到地图中。这需要使用骨架表示的电子密度，这提高了确定的初始链跟踪。片段匹配可用于在模拟同源蛋白质时桥接插入残基的缺口。
Retinol binding protein can be constructed from a small number of large substructures taken from three unrelated proteins. The known structures are treated as a knowledge base from which one extracts information to be used in molecular modelling when lacking true atomic resolution. This includes the interpretation of electron density maps and modelling homologous proteins. Models can be built into maps more accurately and more quickly. This requires the use of a skeleton representation for the electron density which improves the determination of the initial chain tracing. Fragment-matching can be used to bridge gaps for inserted residues when modelling homologous proteins.