In vitro polymerization of complex cytoplasmic gels
In vitro polymerization of complex cytoplasmic gels
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复杂细胞质凝胶的体外聚合
DOI：
10.1021/bk-1987-0350.ch015
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
R. Nossal
中科院分区：
文献类型：
--
作者：
R. Nossal
得到了与细胞质聚合具有相似特征的理论模型的动力学方程的解。研究了各种因素对网络形成和弹性的影响，包括链解离、封盖蛋白的活性、初始单体浓度和交联蛋白的存在。分析了用于研究重建细胞质网络性质的测定方法，并研究了有效使用凝胶分数测定、凝胶时间测定和弹性测量的标准。
Solutions are obtained to kinetic equations that pertain to a theoretical model having features similar to those of polymerizing cytoplasm. The effects of various factors on network formation and elasticity, including chain dissociation, the activity of capping proteins, initial monomer concentration, and the presence of crosslinking proteins are investigated. Assays for studying properties of reconstituted cytoplasmic networks are analyzed, and criteria for effective use of gel fraction determinations, gelation time assays, and elasticity measurements are investigated.