Regions of FtsZ important for self-interaction in Staphylococcus aureus.
Regions of FtsZ important for self-interaction in Staphylococcus aureus.
复制标题
FtsZ 区域对于金黄色葡萄球菌的自我相互作用很重要。
DOI：
10.1006/bbrc.2001.5008
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
D. Payne
中科院分区：
文献类型：
--
作者：
K. Yan;T. Sossong;D. Payne
由于FtsZ在结构上与真核微管蛋白相似，因此S.金黄色葡萄球菌FtsZ已经与来自微管蛋白的等同区域交换。使用酵母双杂交测定分析这些突变FtsZ蛋白与野生型FtsZ和FtsA两者的相互作用。鉴定了废除FtsZ-FtsZ结合但不影响FtsA-FtsZ相互作用的特定区域。这项工作将进一步我们的FtsZ的区域的理解，其生理作用的关键。
Since FtsZ is structurally similar to eukaryotic tubulin, selective regions of S. aureus FtsZ have been swapped with equivalent regions from tubulin. These mutant FtsZ proteins were analyzed for their interaction with both wildtype FtsZ and FtsA using the yeast two-hybrid assay. Specific regions were identified which abrogated FtsZ-FtsZ binding but unaffected the FtsA-FtsZ interaction. This work will further our understanding of the regions of FtsZ critical for its physiological role.