The Caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs
The Caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs
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DOI：
10.1016/s1534-5807(03)00127-8
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发表时间：
2003-05-01
影响因子：
11.8
通讯作者：
Rougvie, AE
中科院分区：
文献类型：
--
作者：
Abrahante, JE;Daul, AL;Rougvie, AE
发育的时间控制是模式形成的一个重要方面，有待完整的分子分析。我们确认lin-57是C.线虫异时基因途径，确保胚后发育事件的适当时机。lin-57功能的丧失导致下皮层终末分化并过早获得成人特征。lin-57是hbl-1，揭示了蠕虫hunchback同系物在控制发育时间中的作用。重要的是，苍蝇驼背（hb）暂时指定神经系统中的细胞命运。hbl-1/lin-57 3 'UTR是下皮和神经系统中胚后下调所必需的，并且包含多个推定的时间调节microRNA（包括let-7）的结合位点。事实上，我们发现hbl-1/lin-57受let-7调节，至少在神经系统中是这样。对hb 3 'UTR的检查揭示了已知苍蝇miRNAs的潜在结合位点。因此，hunchback基因的进化保守可能包括细胞命运规范和microRNA介导的调节的时间控制。
Temporal control of development is an important aspect of pattern formation that awaits complete molecular analysis. We identified lin-57 as a member of the C. elegans heterochronic gene pathway, which ensures that postembryonic developmental events are appropriately timed. Loss of lin-57 function causes the hypodermis to terminally differentiate and acquire adult character prematurely. lin-57 is hbl-1, revealing a role for the worm hunchback homolog in control of developmental time. Significantly, fly hunchback (hb) temporally specifies cell fates in the nervous system. The hbl-1/lin-57 3'UTR is required for postembryonic downregulation in the hypodermis and nervous system and contains multiple putative binding sites for temporally regulated microRNAs, including let-7. Indeed, we find that hbl-1/lin-57 is regulated by let-7, at least in the nervous system. Examination of the hb 3'UTR reveals potential binding sites for known fly miRNAs. Thus, evolutionary conservation of hunchback genes may include temporal control of cell fate specification and microRNA-mediated regulation.