Comparative analysis of foetal calf and human low density lipoprotein: relevance for pharmacodynamics of photosensitizers
Comparative analysis of foetal calf and human low density lipoprotein: relevance for pharmacodynamics of photosensitizers
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DOI：
10.1016/s1011-1344(02)00241-5
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发表时间：
2002-04-01
影响因子：
5.4
通讯作者：
Moore, JV
中科院分区：
文献类型：
--
作者：
Haylett, AK;Moore, JV
研究了不同脂蛋白含量对新型疏水光敏剂正丁基-3-[18-（2-丁基氨基甲酰乙基）-3,7,12,17-四甲基-18,13-二乙烯基-22,24-二氢卟啉-2-基]丙酰胺（PP-N-3）和相对亲水光敏剂血红卟啉酯（HpE）在人（HS）和胎牛血清（FCS）中分布的影响。利用人成纤维细胞系（Vag 12）对人和胎牛低密度脂蛋白（LDL）的结合特性进行了表征。同时检测HpE和PP-N-3的摄取情况。还比较了胎牛和人血清的脂蛋白含量、组成和受体结合特性。用序贯超离心法直接测定LDL含量，分离LDL。在我们的研究中，我们发现血卟啉酯与人极低密度脂蛋白（VLDL）、LDL和高密度脂蛋白（HDL）的结合比例为2:31:65。在PP-N-3的情况下，这个比例是56:10:33。由于在胎牛血清中未检测到VLDL，只观察到与LDL和HDL的结合。利用序贯超离心技术，发现胎牛血清中含有LDL，而LDL又与人LDL受体结合。胎牛血清低密度脂蛋白（FC-LDL）存在时，PP-N-3和HpE的摄取与人血清低密度脂蛋白（HS-LDL）存在时观察到的值没有显著差异。(C) 2002 Elsevier Science B.V.版权所有
The effects of differences in lipoprotein content on the distribution of the novel hydrophobic photosensitizer n-butyl-3-[18-(2-butylcarbamoyl-ethyl)-3,7,12,17-tetramethyl-18,13-divinyl-22,24-dihydro-porphin-2-yl]propionamide (PP-N-3) and haematoporphyrin ester (HpE), a relatively hydrophilic photosensitizer, in human (HS) and foetal calf sera (FCS), were investigated. The binding characteristics of human and foetal calf low-density lipoprotein (LDL) were characterised using a human fibroblast line (Vag 12). The uptake into cells of HpE and PP-N-3 was also examined. A comparison of the lipoprotein content, composition and receptor-binding characteristics of foetal calf and human serum was also carried out. LDL content was measured directly using sequential ultracentrifugation to isolate LDL. In our study, we found haematoporphyrin ester to bind to human very low-density lipoprotein (VLDL), LDL and high-density lipoprotein (HDL) in the ratio 2:31:65. In the case of PP-N-3 this ratio was 56:10:33. As VLDL was not detected in foetal calf serum, only binding to LDL and HDL was observed. Using the sequential ultracentrifugation technique, foetal calf serum was found to contain LDL which in turn did bind to human LDL receptors. The uptake of PP-N-3 and HpE in the presence of low density lipoprotein from foetal calf serum (FC-LDL) was not significantly different to values observed in the presence of human serum low density lipoprotein (HS-LDL). (C) 2002 Elsevier Science B.V. All rights reserved.