Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue
Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue
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DOI：
10.1634/stemcells.2005-0342
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发表时间：
2006-05-01
期刊：
影响因子：
5.2
通讯作者：
Bieback, Karen
中科院分区：
文献类型：
--
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
间充质干细胞（MSC）代表了支持细胞治疗的新临床概念的有前途的工具。骨髓（BM）是报道含有MSC的第一个来源。然而，对于临床应用，BM可能是有害的，这是由于高度侵入性的捐赠程序以及MSC数量和分化潜力随着年龄的增长而下降。最近，通过微创方法获得的脐带血（UCB）被引入作为MSC的替代来源。另一个有希望的来源是脂肪组织（AT）。我们比较了从这些来源获得的MSC的形态、分离MSC的成功率、集落频率、扩增潜力、多分化能力和免疫表型。从这些来源获得的MSC的形态学和免疫表型没有显著差异。骨髓间充质干细胞的分离成功率为100%，而脐带血为63%。殖民地频率在UCB中最低，在AT中最高。UCB-MSCs培养时间最长，增殖能力最强; BM-MSCs培养时间最短，增殖能力最弱。最引人注目的是，UCB-MSC没有表现出成脂分化能力，与BM-和AT-MSC相反。在分离MSC时，UCB和AT都是BM的有吸引力的替代品：AT含有最高频率的MSC，而UCB似乎可以扩展到更高的数量。
Mesenchymal stem cells (MSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. Bone marrow (BM) was the first source reported to contain MSCs. However, for clinical use, BM may be detrimental due to the highly invasive donation procedure and the decline in MSC number and differentiation potential with increasing age. More recently, umbilical cord blood (UCB), attainable by a less invasive method, was introduced as an alternative source for MSCs. Another promising source is adipose tissue (AT). We compared MSCs derived from these sources regarding morphology, the success rate of isolating MSCs, colony frequency, expansion potential, multiple differentiation capacity, and immune phenotype. No significant differences concerning the morphology and immune phenotype of the MSCs derived from these sources were obvious. Differences could be observed concerning the success rate of isolating MSCs, which was 100% for BM and AT, but only 63% for UCB. The colony frequency was lowest in UCB, whereas it was highest in AT. However, UCB-MSCs could be cultured longest and showed the highest proliferation capacity, whereas BM-MSCs possessed the shortest culture period and the lowest proliferation capacity. Most strikingly, UCB-MSCs showed no adipogenic differentiation capacity, in contrast to BM- and AT-MSCs. Both UCB and AT are attractive alternatives to BM in isolating MSC: AT as it contains MSCs at the highest frequency and UCB as it seems to be expandable to higher numbers.