Synthesis of a pentekontapeptide with high lipolytic activity corresponding to the carboxyl-terminal fifty amino acids of ovine beta-lipotropin.
Synthesis of a pentekontapeptide with high lipolytic activity corresponding to the carboxyl-terminal fifty amino acids of ovine beta-lipotropin.
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具有高脂肪分解活性的五肽的合成，对应于绵羊β-促脂素的羧基端五十个氨基酸。
DOI：
10.1073/pnas.71.12.4945
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发表时间：
1974
影响因子：
11.1
通讯作者：
C. Li
中科院分区：
文献类型：
--
作者：
D. Yamashiro;C. Li
用固相法合成了绵羊β-促脂蛋白42-91位的50个氨基酸残基的多肽。完全采用了预成对称酸酐和活性酯偶联法。合成产物经凝胶过滤、羧甲基纤维素层析、Sephadex G-50分配层析纯化。其在分离的兔脂肪细胞中的脂解活性按重量计算约为β-促脂激素的6倍。
The synthesis of a peptide, composed of fifty amino-acid residues, corresponding to positions 42-91 in ovine beta-lipotropin has been accomplished by the solidphase method. The preformed symmetrical anhydride and active ester coupling methods were used exclusively. The synthetic product was purified by gel filtration, carboxymethylcellulose chromatography, and partition chromatography on Sephadex G-50. Its lipolytic activity in isolated rabbit fat cells was about six times that of beta-lipotropin on a weight basis.