Time course of alterations after olfactory bulbectomy in mice
Time course of alterations after olfactory bulbectomy in mice
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DOI：
10.1016/j.physbeh.2006.08.003
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发表时间：
2006-12-30
影响因子：
2.9
通讯作者：
Caretta, Antonio
中科院分区：
文献类型：
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作者：
Mucignat-Caretta, Carla;Bondi, Michela;Caretta, Antonio
啮齿类动物的嗅球切除会导致行为改变，从而产生一种抑郁症模型，这种模型可以用于抗抑郁药物的药理学筛选。为了揭示手术后主要行为影响的外观和时间过程，对小鼠进行嗅球消融或假手术，并在1、2和4周后进行分析。在强迫游泳测试中，在压力情况下进行测试时，球切除（BX）小鼠嗅觉丧失，并且过度活跃。掠食性攻击以时间依赖的方式上调：仅在4周后，BX小鼠攻击猎物的速度比对照组快。与此同时，它们对入侵者的攻击性更低；他们在野外探索中与对照组没有差异，但在水迷宫中表现出认知障碍。因此，行为测试显示出明显的过度反应，不同攻击行为之间的分离，以及球切引起的认知障碍。损伤的组织学证实显示，在手术后2周，额叶吻侧极发生了主要的改变，吻侧迁移流的宽度显著增加，在手术后4周，在脑室下区。这些结果为BX小鼠行为症状出现的时间过程提供了基础。(c) 2006爱思唯尔公司版权所有。
Olfactory bulbectomy in rodents causes behavioral alterations, which result in a model of depression, validated for pharmacological screening of antidepressant drugs. To unravel the appearance and time course of the major behavioral effects which follow surgery, mice underwent olfactory bulb ablation or sham operation, and were analyzed after 1, 2, or 4 weeks. Bulbectomized (BX) mice were anosmic, and hyperactive when tested under stressful situations in the forced swimming test. Predatory aggression was upregulated in a time-dependent way: only after 4 weeks BX mice were faster than controls in attacking prey. At the same time, they were less aggressive against intruders; they did not differ from controls in open field exploration, but displayed a cognitive impairment in water maze. Behavioral tests thus indicated a marked hyperreactivity, a dissociation among different aggressive behaviors, and also a cognitive impairment induced by bulbectomy. Histological confirmation of the damage revealed that major modifications took place in the rostral pole of frontal lobes, with a significant increase in the width of the rostral migratory stream, 2 weeks after surgery, and in the subventricular zone, 4 weeks after surgery. These results suggest a base for the time-course of appearance of behavioral symptoms in BX mice. (c) 2006 Elsevier Inc. All rights reserved.