Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle
Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle
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卵泡细胞对卵泡外成熟绵羊卵母细胞成熟和发育能力的影响
DOI：
10.1002/mrd.1120090211
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Moor
中科院分区：
文献类型：
--
作者：
R. B. Staigmiller;R. Moor
在共培养研究中，卵泡细胞在赋予卵母细胞成熟过程中的发育能力方面起着核心作用的假设已经得到了验证。将不同组合的卵母细胞和卵泡细胞与促性腺激素和雌激素在非静态培养系统中培养24小时。通过将卵母细胞移植到受精卵受体母羊，并在12天后或分娩时进行胚胎检查来评估发育能力。
The hypothesis that follicle cells play a central role in endowing the oocyte with developmental competence during maturation has been tested in coculture studies. Oocytes and follicle cells in various combinations were cultured for 24 h with gonadotrophins and estrogen in a nonstatic culture system. Developmental competence was assessed by transfer of oocytes to inseminated receipient ewes followed by embryonic examination 12 days later or at parturition.
Denuded and corona-enclosed oocytes resume meiosis in culture but remain immature and developmentally incompetent. By contrast, oocytes supported by the cumulus and underlying granulosa (cumulus-oocyte complexes) undergo full maturation and normal subsequent embryonic development (42.6% to embryos). Addition of supplementary follicle cells during culture (5 × 106 cells/ml medium) is without beneficial effect on denuded oocytes. However, supplementary cells confer competence on corona-enclosed oocytes (37% to embryos).
A dual cellular requirement for full maturation involving both cell numbers and some direct cell-oocyte contact is highlighted by our experiments. The results demonstrate further that the nonstatic system provides a simple but reliable method of producing large numbers of fully matured oocytes for both research and clinical purposes.