Development of HPLC analytical protocols for quantification of artemisinin in biomass and extracts
Development of HPLC analytical protocols for quantification of artemisinin in biomass and extracts
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DOI：
10.1016/j.jpba.2009.01.025
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发表时间：
2009-05-01
影响因子：
3.4
通讯作者：
Chemat, Smain
中科院分区：
文献类型：
--
作者：
Lapkin, Alexei A.;Walker, Adam;Chemat, Smain
迄今为止，对植物材料和提取物中青蒿素纯度和数量的定量的特点是数值相当不一致。这可能是由于采用了不同的分析方法和使用了不适合具体应用的分析技术。在本文中，我们试图进一步发展青蒿素分析，使研究和最终用户群体能够获得普遍接受的参考方法。因此，我们开发并验证了HPLC-RI方法，并优化了HPLC-ELSD方法。我们使用当前青蒿素专论推荐的梯度HPLC-UV方法作为本文提出的方法改进的比较，并显示其应用范围的局限性。报告的数据应有助于对纯样品和青蒿提取物中的青蒿素进行更可靠的实验室分析。(c)2009爱思唯尔有限公司版权所有。
Quantification of artemisinin purity and amount in plant material and extracts to date has been characterized by a considerable inconsistency in values. This is likely to be due to the adoption of varied analytical procedures and use of inappropriate to the specific applications analytical techniques. In this paper we are attempting to further develop artemisinin analysis to the point where a universally acceptable reference method is available to the research and end-users communities. Thus, we have developed and validated an HPLC-RI method and optimized an HPLC-ELSD method. We used the gradient HPLC-UV method recommended by the current artemisinin monograph as a comparison for the method improvements presented herein, and show the limitations for its application scope. The data reported should help to allow more reliable laboratory analysis of artemisinin in both pure samples and in Artemisia annua extracts. (c) 2009 Elsevier B.V. All rights reserved.