Differential potential for envelope gycoprotein-mediated steric shielding of host cell surface proteins among filoviruses
Differential potential for envelope gycoprotein-mediated steric shielding of host cell surface proteins among filoviruses
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丝状病毒之间包膜糖蛋白介导的宿主细胞表面蛋白的空间屏蔽的差异潜力
DOI：
10.1016/j.virol.2013.07.029
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Noyori Osamu
中科院分区：
文献类型：
--
作者：
Simulundu E;Nao N;Yabe J;Muto NA;Sithebe T;Sawa H;Manzoor R;Kajihara M;Muramatsu M;Ishii A;Ogawa H;Mweene AS;Takada A;Changula Katendi;Muramatsu M. et al.;Noyori Osamu
病毒包膜糖蛋白(GP)被认为在丝状病毒感染的发病机制中起重要作用。已知，细胞表面表达的GP在宿主蛋白如主要组织相容性复合体I和整合素β1上形成立体屏障，可能导致细胞与细胞之间接触的紊乱和/或免疫反应的抑制。然而，目前还不清楚这种现象是否与丝状病毒的致病性有关。在本研究中，我们发现不同丝状病毒株的立体屏蔽效率不同，并且与它们的相对致病性的差异有关。虽然糖蛋白的高度糖基化的粘蛋白样区是必不可少的，但差异化的屏蔽效率并不一定取决于粘蛋白样区的一级结构，这表明GP分子的整体性质(例如，灵活性和稳定性)对于有效地屏蔽宿主蛋白非常重要。
The viral envelope glycoprotein (GP) is thought to play important roles in the pathogenesis of filovirus infection. It is known that GP expressed on the cell surface forms a steric shield over host proteins such as major histocompatibility complex class I and integrin β1, which may result in the disorder of cell-to-cell contacts and/or inhibition of the immune response. However, it is not clarified whether this phenomenon contributes to the pathogenicity of filoviruses. In this study, we found that the steric shielding efficiency differed among filovirus strains and was correlated with the difference in their relative pathogenicities. While the highly glycosylated mucin-like region of GP was indispensable, the differential shielding efficiency did not necessarily depend on the primary structure of the mucin-like region, suggesting the importance of the overall properties (e.g., flexibility and stability) of the GP molecule for efficient shielding of host proteins.