Dietary garcinol inhibits 4-nitroquinoline 1-oxide-induced tongue carcinogenesis in rats
Dietary garcinol inhibits 4-nitroquinoline 1-oxide-induced tongue carcinogenesis in rats
复制标题
DOI：
10.1016/j.canlet.2004.08.016
复制
发表时间：
2005-04-18
期刊：
影响因子：
9.7
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Yoshida, K;Tanaka, T;Mori, H
在雄性 F344 大鼠中研究了从 Garcinia indica 果皮中分离出的聚异戊二烯二苯甲酮（garcinol）的膳食喂养对 4-硝基喹啉 1-氧化物 (4-NQO) 诱导的口腔癌发生的影响。 7 周龄时，动物在饮用水中添加 20 ppm 的 4-NQO，持续 8 周以诱发舌肿瘤。他们还在接触致癌物期间（10 周）或之后（22 周）接受了含有 100 或 500 ppm 藤黄醇的饮食。其余大鼠在整个实验过程中饮用不含4-NQO的自来水，并饲喂含有山竹酚（500 ppm）的饮食或单独的基础饮食。研究结束时（第32周），舌肿瘤和癌前病变的发生率、通过5-溴脱氧尿苷（BrdU）标记指数和细胞周期蛋白D1阳性细胞比例估计的正常舌上皮中的细胞增殖活性以及舌病变中环氧合酶-2（COX-2）的免疫组织化学表达情况如下： 决定。与对照饮食相比，膳食藤黄醇显着降低了 4-NQO 诱导的舌肿瘤和/或肿瘤前期的发生率和多重性。饮食中给予藤黄酚还显着降低了 BrdU 标记指数和细胞周期蛋白 D1 阳性细胞比例，表明藤黄酚降低了舌头中的细胞增殖活性。喂食藤黄醇也可抑制舌头病变中的 COX-2 表达。这些结果表明，饮食中给予山竹酚通过抑制靶组织中增加的细胞增殖活性和/或舌病变中COX-2的表达来抑制4-NQO诱导的舌癌发生。 (c) 2004 Elsevier Ireland Ltd. 保留所有权利。
The effects of dietary feeding with a polyisoprenylated benzophenone, garcinol, isolated from Garcinia indica fruit rind on the development of 4-nitroquinoline 1-oxide (4-NQO)-induced oral carcinogenesis were investigated in male F344 rats. At 7 weeks of age, animals were given 4-NQO at 20 ppm in the drinking water for 8 weeks to induce tongue neoplasms. They also received the diets containing 100 or 500 ppm garcinol either during (for 10 weeks) or after (for 22 weeks) the carcinogen exposure. The other rats were given tap water without 4-NQO throughout the experiment, and fed garcinol (500 ppm)containing diet or basal diet alone. At the end of the study (week 32), incidences of tongue neoplasms and preneoplastic lesions, cell proliferation activity in the normal-like tongue epithelium estimated by 5-bromodeoxyurideine (BrdU)-labeling index and cyclin D1-positive cell ratio, and immunohistochemical expression of cyclooxygenase-2 (COX-2) in the tongue lesions were determined. Dietary garcinol significantly decreased the incidence and multiplicity of 4-NQO-induced tongue neoplasms and/or preneoplasms as compared to the control diet. Dietary administration of garcinol also significantly reduced the BrdU-labeling index and cyclin D1-positive cell ratio, suggesting reduction in cell proliferation activity in the tongue by garcinol. The COX-2 expression in the tongue lesions was also suppressed by feeding with garcinol. These results indicate that dietary administration of garcinol inhibited 4-NQO-induced tongue carcinogenesis through suppression of increased cell proliferation activity in the target tissues and/or COX-2 expression in the tongue lesions. (c) 2004 Elsevier Ireland Ltd. All rights reserved.