Differentiation of transplanted mesenchymal stem cells in a large osteochondral defect in rabbit
Differentiation of transplanted mesenchymal stem cells in a large osteochondral defect in rabbit
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DOI：
10.1080/14653240500361350
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发表时间：
2005-12-01
期刊：
影响因子：
4.5
通讯作者：
Ueda, M
中科院分区：
文献类型：
--
作者：
Tatebe, M;Nakamura, R;Ueda, M
背景虽然越来越多的证据表明间充质干细胞（MSC）是关节软骨修复的有前途的细胞来源，但移植的MSC的命运尚未得到广泛研究。方法为了监测其持久性和分化，我们用荧光染料PKH26标记未诱导的MSC，并将它们移植到聚乙醇酸支架中，用透明质酸移植到兔股骨滑车承重区域的全层缺损处床单。对标记细胞的命运进行长达 8 周的监测。结果移植两周后，含有 II 型胶原蛋白的未成熟软骨形成。 8周时，软骨变薄，II型胶原蛋白的免疫标记逐渐从基底区域消失，I型胶原蛋白呈阳性。再生软骨内的大多数软骨细胞是PKH26阳性，因此源自移植的MSC，而再生骨内的成骨细胞是供体和宿主来源细胞的混合物。软骨厚度在术后 8 周内变薄，然后保持稳定长达 42 周。讨论这些结果表明，未诱导的 MSC 能够在骨软骨缺损中存活并根据环境进行分化，对初始软骨形成做出主要贡献，并对骨再生做出部分贡献。
BackgroundAlthough accumulating evidence shows that mesenchymal stem cells (MSC) are a promising cell source for articular cartilage repair, the fate of transplanted MSC has not been extensively studied.MethodsTo monitor their persistence and differentiation, we labeled uninduced MSC with a fluorescent dye, PKH26, and transplanted them, in a poly-glycolic-acid scaffold, to full-thickness defects made in the weight-bearing area of rabbit femoral trochleae with hyaluronate sheets. The fate of the labeled cells was monitored for up to 8 weeks.ResultsTwo weeks after transplantation, immature cartilage containing collagen type II had formed. By 8 weeks, this cartilage had thinned and immunolabeling for collagen type II gradually disappeared from the basal region, which became positive for collagen type I. Most chondrocytes within the regenerated cartilage were PKH26-positive and, therefore, derived from transplanted MSC, whereas osteoblasts within the regenerated bone were a mixture of donor- and host-derived cells. The thickness of the cartilage became thinner up to 8 weeks and then remained stable up to 42 weeks after surgery.DiscussionThese results showed that uninduced MSC were able to survive osteochondral defects and differentiated according to the environment, making a major contribution to initial cartilage formation and a partial contribution to bone regeneration.