Prevalence of Wolbachia Supergroups A and B in Bemisia tabaci (Hemiptera: Aleyrodidae) and Some of Its Natural Enemies
Prevalence of Wolbachia Supergroups A and B in Bemisia tabaci (Hemiptera: Aleyrodidae) and Some of Its Natural Enemies
复制标题
烟粉虱（半翅目：粉虱科）及其一些天敌中沃尔巴克氏体超群 A 和 B 的流行情况
DOI：
10.1603/ec10033
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发表时间：
2010-10-01
影响因子：
2.2
通讯作者：
Qiu, Bao-Li
中科院分区：
文献类型：
--
作者：
Ahmed, Muhammad Z.;Ren, Shun-Xiang;Qiu, Bao-Li
沃尔巴克氏菌是一种细菌共生体，通过节肢动物和线虫通过母体传播。本文报道了甘薯烟粉虱Bomesia Tabaci(Gennadius)(半翅目：粉虱科)及其部分天敌的Wolbachia分类研究。首次将烟粉虱体内的沃尔巴克氏菌感染与不同的烟粉虱基因群、寄主植物和天敌以及地理区域相关联。利用16S rDNA和FtsZ基因的聚合酶链式反应发现，在烟粉虱及其一些姬蜂和捕食性甲虫中，两个沃尔巴克氏菌超群A和B以单感染或双感染的形式存在。约89%的烟粉虱被沃尔巴克氏菌感染，其中34%被A感染，51%被B感染，5%同时被A和B超群感染。这些感染频率在烟粉虱遗传群体和地点之间存在差异。来自中东小亚细亚1号(又称B生物型)和地中海(又称Q生物型)的烟粉虱入侵种群携带B的可能性大于A，而亚洲1号和亚洲II的本地遗传种群携带B的可能性大于A。虽然60%的姬蜂和72%的瓢虫也感染沃尔巴克氏菌，但它们更有可能宿主B而不是A。此外，我们首次从中国外采集的标本中报道了B生物型的沃尔巴克氏菌。系统发育树的构建清楚地表明，烟粉虱不同遗传群体的Wolbachia序列不仅彼此相似，而且与甲虫和寄生蜂的序列也很相似，这可能为Wolbachia种群的共同进化和水平传播提供证据。
Wolbachia, a bacterial symbiont, is maternally transmitted in arthropods and nematodes. We report a systematic survey of Wolbachia taxonomy in the sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), and in some of its natural enemies. For the first time, Wolbachia infections in B. tabaci are correlated with various whitefly genetic groups, host plants, and natural enemies as well as with geographical regions. Polymerase chain reaction using 16S rDNA and ftsZ genes revealed two Wolbachia supergroups, A and B, exist as single or double infections in B. tabaci as well as in some of its aphelinid parasitoids and predatory beetles. Approximately 89% of B. tabaci sampled were infected by Wolbachia, among which 34% were infected by A, 51% were infected by B, and 5% were infected by both A and B supergroups. These infection frequencies differed among B. tabaci genetic groups and locations. The invasive B. tabaci genetic group from the Middle East Asia Minor 1 (also referred as B biotype) and Mediterranean (also referred as Q biotype) was more likely to harbor A than B, whereas native genetic groups in AsiaI and AsiaII were more likely to harbor B than A. Although 60% of aphelinid parasitoids and 72% of coccinellid beetles also were infected by Wolbachia, they were more likely to host B than A. Furthermore, for the first time we report Wolbachia in B biotype from specimens collected outside of China. Construction of a phylogenetic tree clearly indicated that the Wolbachia sequences from different genetic groups of B. tabaci were not only similar to each other but also to sequences from beetles and parasitoids, which may provide evidence of coevolution and horizontal transmission of Wolbachia populations.