Sexual dimorphic expression of DMRT1 and Sox9a during gonadal differentiation and hormone-induced sex reversal in the teleost fish nile tilapia (Oreochromis niloticus)
Sexual dimorphic expression of DMRT1 and Sox9a during gonadal differentiation and hormone-induced sex reversal in the teleost fish nile tilapia (Oreochromis niloticus)
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DOI：
10.1002/dvdy.21409
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Nagahama, Yoshitaka
中科院分区：
文献类型：
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作者：
Kobayashi, Tohru;Kajiura-Kobayashi, Hiroko;Nagahama, Yoshitaka
我们检查了性腺性别分化和激素诱导的性别逆转过程中 tDMRT1 和 Sox9a 的表达谱。 tDMRT1 在 XY 鱼苗的生殖细胞周围细胞中检测到，特别是在任何形态性别分化迹象出现之前，即生殖细胞数量和组织发生的性别差异，例如分化为睾丸内传出管或卵巢腔。在性腺分化过程中，信号定位于构成传出管的支持细胞和上皮细胞中。用雌激素诱导XY性逆转后，tDMRT1下降，然后完全消失。相比之下，在雄激素导致的 XX 性逆转中，tDMRT1 在生殖细胞周围的细胞中表达。另一方面，Sox9a在组织发生中出现性别差异之前并不表现出性别二态性，并且在睾丸传出管中不表达。这些结果表明 tDMRT1 是罗非鱼的优良睾丸分化标记物。发育动力学 237:297-306, 2008。(c) 2007 Wiley-Liss, Inc.
We examined the expression profiles of tDMRT1 and Sox9a during gonadal sex differentiation and hormone-induced sex reversal. tDMRT1 was detected in the gonial germ-cell-surrounding cells in XY fry specifically before the appearance of any signs of morphological sex differentiation, that is, sex differences in germ cell number and histogenesis, such as differentiation into intratesticular efferent duct or ovarian cavity. The signals became localized in the Sertoli and epithelial cells comprising the efferent duct during gonadal differentiation. After the induction of XY sex reversal with estrogen, tDMRT1 decreased and then disappeared completely. In contrast, tDMRT1 was expressed in the germ-cell-surrounding cells in XX sex reversal with androgen. On the other hand, Sox9a did not show sexual dimorphism before the appearance of sex differences in histogenesis and was not expressed in the efferent duct in the testis. These results suggest that tDMRT1 is a superior testicular differentiation marker in tilapia. Developmental Dynamics 237:297-306, 2008. (c) 2007 Wiley-Liss, Inc.