Expression of AE2 anion exchanger in mouse intestine
Expression of AE2 anion exchanger in mouse intestine
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DOI：
10.1152/ajpgi.1999.277.2.g321
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发表时间：
1999-08-01
影响因子：
4.5
通讯作者：
Seidler, U
中科院分区：
文献类型：
--
作者：
Alper, SL;Rossmann, H;Seidler, U
我们的特点表达阴离子交换器2（AE 2）的mRNA和蛋白质在小鼠肠道。AE 2 mRNA丰度在结肠中高于更近端的节段。在整个肠道中，AF 2a mRNA比AE 2b mRNA更丰富，而AE 2c mRNA以非常低的水平表达。与小鼠胃中AE 2c> AE 2b> AE 2a的mRNA表达模式不同，小鼠胃中AE 2c> AE 2b> AE 2a。免疫印迹法检测到的AE 2多肽丰度与mRNA的丰度定性一致，而AE 2免疫染色显示从结肠到十二指肠强度更连续地降低。AE 2多肽在结肠表面细胞中比在隐窝中更丰富，而回肠隐窝和绒毛表现出相似的AE 2丰度。AE 2也见于壁和血管平滑肌。AE 2表位的定位仅限于整个肠道上皮细胞的基底外侧膜，有三个例外。在温和的固定条件下，抗AE 2氨基酸（aa）109-122检测回肠肠上皮细胞和潘氏细胞颗粒膜的非极化免疫染色。由抗AE 2 aa 1224-1237检测到的表位也定位于十二指肠和空肠上部的Brunner腺管的近顶端区域。这些本地化的研究将有助于解释阴离子交换功能的上皮片，分离的细胞，膜囊泡测量。
We have characterized expression of anion exchanger 2 (AE2) mRNA and protein in the mouse intestine. AE2 mRNA abundance was higher in colon than in more proximal segments. AF2a mRNA was more abundant than AE2b mRNA throughout the intestine, and AE2c mRNA was expressed at very low levels. This AE2 mRNA pattern contrasted with that in mouse stomach, in which AE2c > AE2b > AE2a. AE2 polypeptide abundance as detected by immunoblot qualitatively paralleled that of mRNA, whereas AE2 immunostaining exhibited a more continuous decrease in intensity from colon to duodenum. AE2 polypeptide was more abundant in colonic surface cells than in crypts, whereas ileal crypts and villi exhibited similar AE2 abundance. AE2 was also observed in mural and vascular smooth muscle. Localization of AE2 epitopes was restricted to the basolateral membranes of epithelial cells throughout the intestine with three exceptions. Under mild fixation conditions, anti-AE2 amino acids (aa) 109-122 detected nonpolarized immunostaining of ileal enterocytes and of Paneth cell granule membranes. An epitope detected by anti-AE2 aa 1224-1237 was also localized to subapical regions of Brunner's gland ducts of duodenum and upper jejunum. These localization studies will aid in the interpretation of anion exchanger function measured in epithelial sheets, isolated cells, and membrane vesicles.