Increased volatile anesthetic requirement in short-sleeping Drosophila mutants.
Increased volatile anesthetic requirement in short-sleeping Drosophila mutants.
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DOI：
10.1097/aln.0b013e3181942df2
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发表时间：
2009-02
期刊：
影响因子：
8.8
通讯作者：
Bein B
中科院分区：
文献类型：
--
作者：
Weber B;Schaper C;Bushey D;Rohlfs M;Steinfath M;Tononi G;Cirelli C;Scholz J;Bein B
麻醉和睡眠在生理和行为上有相似之处。对最近发现的果蝇突变体小潜蝇和其他果蝇突变体的麻醉需求进行了研究。通过测量个体野生型和突变型果蝇的活动来确定睡眠和觉醒。根据果蝇对不同浓度的挥发性麻醉药异氟烷和七氟烷的反应，绘制浓度-反应曲线，计算EC 50值。野生型果蝇（n=64）的平均每日睡眠时间为965 ±15分钟，na[har 38]为1022 ± 29分钟，p>0.05; n=32）（平均值± SEM，与野生型和其他shaker等位基因相比均为p）。Shmns果蝇睡眠时间为584 ±13分钟（n=64，p<0.01），Sh 102为412 ± 22分钟（n=32，p<0.01），Sh 120为782 ± 25分钟（n=32，p<0.01）。异氟烷的EC 50值为0.706（95%置信区间0.649 - 0.764，n=661），七氟烷为1.298（1.180至1.416，n=522）野生型果蝇，1.599（1.527至1.671，n=308）和2.329（2.177至2.482，n=282），在Sh 102中，1.306（1.212至1.400，n=393）和2.013（1.868 - 2.158，n=550），Shmns，0.957（0.860至1.054，n=297）和1.619在Sh 120中分别为0.6154（0.581至0.649，n =360; p<0.05）和0.9339（0.823至1.041，n= 274）（均p<0.01）。果蝇中的一个单基因突变导致日常睡眠极度减少，这是挥发性麻醉剂需求显着增加的原因。这表明，一个单一的基因突变会影响睡眠行为和麻醉和镇静。
Anesthesia and sleep share physiological and behavioral similarities. The anesthetic requirement of the recently identified Drosophila mutant minisleeper and other Drosophila mutants was investigated. Sleep and wakefulness were determined by measuring activity of individual wild-type and mutant flies. Based on the response of the flies at different concentrations of the volatile anesthetics isoflurane and sevoflurane, concentration-response curves were generated and EC50 values were calculated. The average amount of daily sleep in wild-type Drosophila (n=64) was 965 ±15 minutes and 1022 ± 29 in na[har38] p>0.05; n=32) (mean ± SEM, all p compared to wild-type and other shaker alleles). Shmns flies slept 584 ±13 minutes (n=64, p<0.01), Sh102 412 ± 22 minutes (n=32, p<0.01) and Sh120 782 ± 25 minutes (n=32, p<0.01). The EC50 values for isoflurane were 0.706 (95% confidence interval 0.649 to 0.764, n=661) and for sevoflurane 1.298 (1.180 to 1.416, n=522) in wild-type Drosophila, 1.599 (1.527 to 1.671, n=308) and 2.329 (2.177 to 2.482, n=282) in Sh102, 1.306 (1.212 to 1.400, n=393) and 2.013 (1.868 to 2.158, n=550) in Shmns, 0.957 (0.860 to 1.054, n=297) and 1.619 (1.508 to 1.731, n=386) in Sh120, and 0.6154 (0.581 to 0.649, n=360; p<0.05) and 0.9339 (0.823 to 1.041, n= 274) in na[har38], respectively (all p<0.01). A single-gene mutation in Drosophila that causes an extreme reduction in daily sleep is responsible for a significant increase in the requirement of volatile anesthetics. This suggests that a single gene mutation affects both sleep behavior and anesthesia and sedation.