Leptin and vascular endothelial growth factor regulate angiogenesis in tooth germs
Leptin and vascular endothelial growth factor regulate angiogenesis in tooth germs
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DOI：
10.1007/s00418-011-0789-z
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发表时间：
2011-03-01
影响因子：
2.3
通讯作者：
Satomura, Kazuhito
中科院分区：
文献类型：
--
作者：
Ide, Shinji;Tokuyama, Reiko;Satomura, Kazuhito
瘦素是一种由ob基因产生的含有146个氨基酸的16 kDa的非糖基化多肽，不仅在脂质代谢、造血、产热和卵巢功能中具有多种生理作用，而且在血管生成中也具有多种生理作用。本研究旨在探讨瘦素作为一种血管生成因子，在牙齿发育过程中调控血管生成的可能性。我们首先用免疫组织化学方法研究了瘦素和血管内皮生长因子（VEGF）在牙齿发育过程中的表达。瘦素在成釉细胞、成牙细胞、牙乳头细胞和间质层细胞中均有表达。这种表达模式与最有效的血管生成因子之一VEGF相似。有趣的是，与中间和下三分之一相比，在最接近成牙细胞层的牙乳头上三分之一部分观察到更多的瘦素阳性细胞。此外，在牙乳头中，在表达瘦素和VEGF的成釉细胞和成牙细胞附近观察到更多的CD31和/或cd34阳性血管内皮细胞。这些发现强烈提示成釉细胞、成牙细胞和牙乳头细胞通过分泌瘦素和VEGF诱导牙胚血管生成。
Leptin, a 16 kDa non-glycolated polypeptide of 146 amino acids produced by the ob gene, has a variety of physiological roles not only in lipid metabolism, hemato-poiesis, thermogenesis and ovarian function, but also in angiogenesis. This study focuses to investigate the possibility that leptin, as an angiogenic factor, may regulate the angiogenesis during tooth development. We firstly studied the expression of leptin and vascular endothelial growth factor (VEGF) during tooth development immunohistochemically. This investigation revealed that leptin is expressed in ameloblasts, odontoblasts, dental papilla cells and stratum intermedium cells. This expression pattern was similar to that of VEGF, one of the most potent angiogenic factors. Interestingly, more leptin-positive cells were observed in the upper third portion of dental papilla, which is closest to odontoblastic layer, compared to middle and lower thirds. Moreover, in the dental papilla, more CD31 and/or CD34-positive vascular endothelial cells were observed in the vicinity of ameloblasts and odontoblasts expressing leptin and VEGF. These findings strongly suggest that ameloblasts, odontoblasts and dental papilla cells induce the angiogenesis in tooth germs by secretion of leptin as well as VEGF.