Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis
Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis
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DOI：
10.1046/j.1365-313x.2002.01356.x
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发表时间：
2002-08-01
期刊：
影响因子：
7.2
通讯作者：
Smith, FW
中科院分区：
文献类型：
--
作者：
Mudge, SR;Rae, AL;Smith, FW
拟南芥基因组的完成揭示了该物种中磷酸盐转运蛋白Pht 1家族的9个成员。作为鉴定该基因家族在磷营养中的作用的一步，我们已经从这些基因中的每一个分离了启动子区域，并将它们融合到报告基因β-葡萄糖醛酸酶和/或绿色荧光蛋白。这些嵌合基因已被导入A.拟南芥，和报告基因的表达已在含有高和低浓度的无机磷酸盐（Pi）的土壤中生长的植物进行了测定。这些启动子中的四个被发现直接报告基因在根表皮中的表达，并诱导磷酸剥夺的条件下，以类似于以前表征的Pht 1基因的方式。然而，该家族的其他成员在一系列芽组织和花粉粒中表达，这通过RT-PCR证实。我们还提供了证据表明，根表皮表达的基因表达最强烈的毛细胞，摄取的主要网站的Pi。这些结果表明，该基因家族在整个植物的磷酸盐吸收和再动员中起着比以前认为的更广泛的作用。
The completion of the Arabidopsis thaliana genome has revealed that there are nine members of the Pht1 family of phosphate transporters in this species. As a step towards identifying the role of this gene family in phosphorus nutrition, we have isolated the promoter regions from each of these genes, and fused them to the reporter genes beta-glucuronidase and/or green fluorescent protein. These chimeric genes have been introduced into A. thaliana , and reporter gene expression has been assayed in plants grown in soil containing high and low concentrations of inorganic phosphate (Pi). Four of these promoters were found to direct reporter gene expression in the root epidermis, and were induced under conditions of phosphate deprivation in a manner similar to previously characterised Pht1 genes. Other members of this family, however, showed expression in a range of shoot tissues and in pollen grains, which was confirmed by RT-PCR. We also provide evidence that the root epidermally expressed genes are expressed most strongly in trichoblasts, the primary sites for uptake of Pi. These results suggest that this gene family plays a wider role in phosphate uptake and remobilisation throughout the plant than was previously believed.