IN-VIVO ASSESSMENT OF MITOCHONDRIAL FUNCTIONALITY IN HUMAN GASTROCNEMIUS-MUSCLE BY P-31 MRS - THE ROLE OF PH IN THE EVALUATION OF PHOSPHOCREATINE AND INORGANIC-PHOSPHATE RECOVERIES FROM EXERCISE
IN-VIVO ASSESSMENT OF MITOCHONDRIAL FUNCTIONALITY IN HUMAN GASTROCNEMIUS-MUSCLE BY P-31 MRS - THE ROLE OF PH IN THE EVALUATION OF PHOSPHOCREATINE AND INORGANIC-PHOSPHATE RECOVERIES FROM EXERCISE
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DOI：
10.1002/nbm.1940060404
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发表时间：
1993-07-01
影响因子：
2.9
通讯作者：
BARBIROLI, B
中科院分区：
文献类型：
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作者：
IOTTI, S;LODI, R;BARBIROLI, B
在这项研究中，我们比较了 38 名正常个体的磷酸肌酸 (PCr) 和 P(i) 恢复动力学及其对细胞质 pH 的依赖性。光谱是在休息、工作和恢复期间采集的。使用 10 秒的时间分辨率来获取详细信息。当恢复过程中达到的胞质 pH 值最低值（称为最小 pH）< 6.95 时，PCr 和 P(i) 恢复动力学几乎重叠，而当最小 pH > 6.95 时，它们完全解离。该结果被解释为 ADP 对线粒体氧化施加动力学控制的间接体内证据。我们的结果代表了新实验条件用于原发性或继发性线粒体功能障碍病理学临床常规研究的基本原理。
In this study we compared the kinetics of phosphocreatine (PCr) and P(i) recovery, and their dependency on cytosolic pH in 38 normal individuals. Spectra were acquired during rest, work and recovery. A time resolution of 10 s was used to obtain detailed information. The kinetics of PCr and P(i) recovery almost overlapped when the lowest value of cytosolic pH reached during recovery (termed the minimum pH) was < 6.95, while they were completely dissociated when the minimum pH was > 6.95. This result is interpreted as indirect in vivo evidence of the kinetic control exerted by ADP on mitochondrial oxidation. Our results represent a rationale for new experimental conditions to be used in clinical routine studies of pathologies due to primary or secondary mitochondrial malfunction.