Efficacy of a respiratory syncytial virus vaccine candidate in a maternal immunization model.
Efficacy of a respiratory syncytial virus vaccine candidate in a maternal immunization model.
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DOI：
10.1038/s41467-018-04216-6
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发表时间：
2018-05-15
影响因子：
16.6
通讯作者：
Morrison TG
中科院分区：
文献类型：
--
作者：
Blanco JCG;Pletneva LM;McGinnes-Cullen L;Otoa RO;Patel MC;Fernando LR;Boukhvalova MS;Morrison TG
呼吸道合胞病毒（RSV）是婴儿毛细支气管炎最常见的病因。孕产妇免疫是提高孕产妇抗体水平和保护婴儿免受感染的一种选择。在此，我们评估了含有RSV F蛋白和附着RSV G蛋白的稳定的融合前（pre-F）或融合后（post-F）构象的病毒样颗粒（VLP）疫苗候选物在使用棉鼠的母体免疫模型中的功效。含有RSV F和G蛋白的VLP疫苗强烈增强母鼠中预先存在的RSV免疫力，防止其围产期免疫力下降。前F VLP的加强比后F VLP或纯化的亚单位F蛋白疫苗更强，对母亲的保护具有优势。母鼠的VLP免疫为幼鼠提供了显著的保护，使其免受RSV攻击并减少肺部炎症。总的来说，我们的结果表明，具有RSV F和G蛋白的VLP疫苗对于母体和成人疫苗接种是安全有效的。RSV感染是婴儿毛细支气管炎的主要原因，母亲接种疫苗是一种潜在的预防选择。在此，布兰科等人显示了基于纽卡斯尔病病毒的病毒样颗粒疫苗候选物在幼稚和预先暴露的棉鼠母鼠及其后代中的功效。
Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants. Maternal immunization is an option to increase maternal antibody levels and protect infants from infection. Here we assess the efficacy of virus-like particle (VLP) vaccine candidates containing stabilized pre-fusion (pre-F) or post-fusion (post-F) conformations of the RSV F protein and the attachment RSV G protein in a maternal immunization model using cotton rats. VLP vaccines containing RSV F and G proteins strongly boost pre-existing RSV immunity in dams preventing their perinatal drop in immunity. Boosting is stronger for the pre-F VLP than for the post-F VLP or purified subunit F protein vaccines, giving an advantage on mothers’ protection. VLP immunization of dams provides significant protection to pups from RSV challenge and reduced pulmonary inflammation. Collectively, our results show that a VLP vaccine with RSV F and G proteins is safe and effective for maternal and adult vaccination. RSV infection is a major cause of bronchiolitis in infants and maternal vaccination is a potential preventive option. Here, Blanco et al. show efficacy of a Newcastle disease virus-based virus-like particle vaccine candidate in naive and pre-exposed cotton rat dams and their offspring.