In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries
In vitro growth and maturation as well as fertilization of mouse preantral oocytes from vitrified ovaries
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DOI：
10.1016/j.fertnstert.2003.08.028
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发表时间：
2004-03-01
影响因子：
6.7
通讯作者：
Koyama, K
中科院分区：
文献类型：
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作者：
Hasegawa, A;Hamada, Y;Koyama, K
目的：目的探讨玻璃化冷冻卵巢卵母细胞-颗粒细胞复合体（OGC）中腔前卵母细胞的受精和发育能力。设计：实验动物研究。地点：大学研究实验室。动物：正常（C57 BL/6xDBA 2）F1小鼠在实验室环境中的实验干预：使用聚酯片作为储存装置玻璃化小鼠卵巢;通过酶处理收集OGC;体外培养（IVG）、体外成熟（IVM）和体外受精（IVF）。主要结果测量：我们对玻璃化冷冻和加温的卵巢进行组织学分析，并测量从卵巢收集的OGC中的卵母细胞在IVG、IVM和IVF中的成功率。卵巢皮质区在玻璃化冷冻和复温后仍保持良好的形态结构。卵母细胞经IVG和IVM后，75.9%的卵母细胞成熟至中期II（MII）。新鲜卵巢的受精率为69.5%，玻璃化冷冻法保存卵巢的受精率为57.5%。封闭在腔前卵泡中的卵母细胞保存了受精和发育为植入前胚胎的能力。((C)2004年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To examine the capacity for fertilization and development of preantral oocytes in oocyte-granulosa cell complexes (OGC) originating from vitrified ovaries.Design: Experimental animal study.Setting:University-based research laboratory.Animal(s): Normal (C57BL/6xDBA2) F1 mice in a laboratory environment.Intervention(s): Vitrification of mouse ovaries using polyester sheets as a storage device; collection of OGC by enzymatic treatment; in vitro growth (IVG), in vitro maturation (IVM), and in vitro fertilization (IVF).Main Outcome Measure(s): We performed histologic analysis of vitrified and warmed ovaries, and measured the successful rate in IVG, IVM, and IVF of oocytes in the OGC collected from the ovaries.Result(s): The cortical region of ovaries maintained good morphologic structure after vitrification and warming. Upon IVG and IVM, 75.9% of oocytes in OGC matured to the metaphase II (MII) stage. The fertilization rate of these oocytes was 57.5% as compared with 69.5% for fresh ovaries.Conclusion(s): The vitrification method used was effective for storage of ovaries. The oocytes enclosed in preantral follicles from the ovaries preserved capacity for fertilization and development to preimplantation embryos. ((C) 2004 by American Society for Reproductive Medicine.)