Insight into the routes of Wolbachia invasion:: high levels of horizontal transfer in the spider genus Agelenopsis revealed by Wolbachia strain and mitochondrial DNA diversity
Insight into the routes of Wolbachia invasion:: high levels of horizontal transfer in the spider genus Agelenopsis revealed by Wolbachia strain and mitochondrial DNA diversity
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DOI：
10.1111/j.1365-294x.2007.03608.x
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发表时间：
2008-01-01
影响因子：
4.9
通讯作者：
Werren, John H.
中科院分区：
文献类型：
--
作者：
Baldo, Laura;Ayoub, Nadia A.;Werren, John H.
沃尔巴克氏体（α-变形菌）在节肢动物中的大流行分布很大程度上是由于这些母系遗传的内共生体成功地跨物种边界转移宿主的能力。然而，目前尚不清楚沃尔巴克氏体是否具有物种间转移的优先途径。在这里，我们检查了北美漏斗网蜘蛛属 Agelenopsis 的八个物种的种群，以评估沃尔巴克氏体是否显示出宿主特异性的证据以及相关宿主物种内和之间菌株水平与垂直传播的相对贡献。通过多位点序列分型 (MLST) 和沃尔巴克氏体表面蛋白 (WSP) 序列对沃尔巴克氏体菌株进行了表征，并分析了与宿主系统发育、线粒体多样性和地理范围的关系。结果表明，至少三组不同的沃尔巴克氏菌菌株入侵了Agelenopsis 属。每次入侵后，沃尔巴克氏菌菌株优先通过水平转移而不是共种在该宿主属的物种之间进行洗牌。沃尔巴克氏体与单个物种内宿主线粒体单倍型（有丝分裂型）进化历史的解耦揭示了水平转移在沃尔巴克氏体在同种宿主群体中快速扩散中的广泛贡献。这些发现提供了一些最有力的证据，支持相关沃尔巴克氏体菌株通过垂直和水平菌株传播与相关宿主之间的关联。需要对更广泛的无脊椎动物类群进行类似的分析，使用诸如 MLST 等敏感的菌株分型方法，以确定沃尔巴克氏体的这种传播模式是否是 Agelenopsis（或蜘蛛）特有的，或者实际上是节肢动物的普遍模式。
The pandemic distribution of Wolbachia (alpha-proteobacteria) across arthropods is largely due to the ability of these maternally inherited endosymbionts to successfully shift hosts across species boundaries. Yet it remains unclear whether Wolbachia has preferential routes of transfer among species. Here, we examined populations of eight species of the North American funnel-web spider genus Agelenopsis to evaluate whether Wolbachia show evidence for host specificity and the relative contribution of horizontal vs. vertical transmission of strains within and among related host species. Wolbachia strains were characterized by multilocus sequence typing (MLST) and Wolbachia surface protein (WSP) sequences, and analysed in relation to host phylogeny, mitochondrial diversity and geographical range. Results indicate that at least three sets of divergent Wolbachia strains invaded the genus Agelenopsis. After each invasion, the Wolbachia strains preferentially shuffled across species of this host genus by horizontal transfer rather than cospeciation. Decoupling of Wolbachia and host mitochondrial haplotype (mitotypes) evolutionary histories within single species reveals an extensive contribution of horizontal transfer also in the rapid dispersal of Wolbachia among conspecific host populations. These findings provide some of the strongest evidence to support the association of related Wolbachia strains with related hosts by means of both vertical and horizontal strain transmission. Similar analyses across a broader range of invertebrate taxa are needed, using sensitive methods for strain typing such as MLST, to determine if this pattern of Wolbachia dispersal is peculiar to Agelenopsis (or spiders), or is in fact a general pattern in arthropods.