Development of multidrug resistance in a canine lymphoma cell line
Development of multidrug resistance in a canine lymphoma cell line
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DOI：
10.1016/j.rvsc.2004.09.012
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发表时间：
2005-06-01
影响因子：
2.4
通讯作者：
Taura, Y
中科院分区：
文献类型：
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作者：
Uozurmi, K;Nakaichi, M;Taura, Y
从犬B细胞淋巴瘤细胞株（GL-1）发展出新的多药耐药细胞株，对一些抗肿瘤药物的化学敏感性和p -糖蛋白（Pgp）的表达进行了表征。将GL-1连续暴露于含有逐渐增加的阿霉素水平的培养基中，获得在阿霉素存在下可以生长的细胞。用维拉帕米或不使用维拉帕米，研究了这些细胞对各种抗肿瘤药物的化学敏感性，维拉帕米逆转了pgp介导的耐药性。Western blot检测Pgp在细胞上的表达。结果获得了3种耐药细胞系，分别为GL-DOX60、300和4000。这些细胞系在含60、300和4000 ng/ml的培养基中增殖稳定。分别。这些细胞对长春新碱的抵抗力比阿霉素强得多。维拉帕米的存在有力地逆转了这种耐药性。另一方面，顺铂在杀死这些衍生细胞方面足够有效。在Western Blot分析中，在GL-DOX4000中观察到一些与抗人Pgp单克隆抗体反应的条带。GL-1衍生的细胞具有犬Pgp介导的多药耐药潜能。在本实验中产生的细胞被认为是研究犬多药耐药性的有用模型。(c) 2004年Elsevier Ltd出版
New multidrug resistant cell lines developed from the canine B cell lymphoma cell line (GL-1) were characterized in terms of chemosensitivity to some antineoplastics and P-glycoprotein (Pgp) expression. GL-1 was continuously exposed to a culture medium containing gradually increasing levels of doxorubicin and the cells that could grow in the presence of doxorubicin were obtained. Chemosensitivity of these cells to various antineoplastics were investigated with or without verapamil, which reversed Pgp-mediated drug resistance. The expression of Pgp on the cells was also examined by Western blot analysis. As a result, three kinds of resistant cell lines, designated as GL-DOX60, 300, and 4000 were obtained. These cell lines showed stable proliferation in the medium containing 60, 300, and 4000 ng/ml. respectively. These cells were much more resistant to vincristine than doxorubicin. This resistance was strongly reversed by the presence of verapamil. On the other hand, cisplatin was effective enough in killing these derived cells. In the Western Blot analysis, some bands that reacted to the anti-human Pgp monoclonal antibodies were observed in GL-DOX4000. The cells derived from GL-1 have multidrug resistance potential mediated by canine Pgp. The cells produced in this experimental trial are considered to be useful models for various investigations on canine multidrug resistance. (c) 2004 Published by Elsevier Ltd.