Prostaglandin E receptor subtype EP3 expression in human conjunctival epithelium and its changes in various ocular surface disorders.
Prostaglandin E receptor subtype EP3 expression in human conjunctival epithelium and its changes in various ocular surface disorders.
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DOI：
10.1371/journal.pone.0025209
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kinoshita S
中科院分区：
文献类型：
--
作者：
Ueta M;Sotozono C;Yokoi N;Inatomi T;Kinoshita S
在我们早期针对史蒂文斯-约翰逊综合征 (SJS) 及其严重变异体中毒性表皮坏死松解症 (TEN) 的全基因组关联研究中，我们发现患有这些严重眼表并发症的日本患者与前列腺素 E 受体 3 (EP3) 基因 (PTGER3) 多态性相关。我们还报道EP3主要在眼表尤其是结膜上皮中表达，并表明结膜上皮中的EP3可能下调眼表炎症。在当前的研究中，我们调查了各种眼表疾病（例如SJS/TEN、化学性眼烧伤、蚕蚀性溃疡和眼部瘢痕性类天疱疮（OCP））患者结膜中EP3蛋白的表达。结膜组织取自因 SJS/TEN、化学眼烧伤和 OCP 而接受眼表手术重建的患者，以及通过切除炎性结膜治疗的蚕蚀性溃疡患者。对照是在结膜松弛手术中获得的几乎正常的人类结膜组织。我们对 EP3 蛋白进行了免疫组织学分析，并评估了眼表疾病患者结膜上皮中 EP3 蛋白的免疫组织学染色情况。 EP3在化学性眼烧伤和蚕蚀性溃疡患者的结膜上皮以及正常人结膜上皮中表达。然而，它在 SJS/TEN 和 OCP 患者的结膜上皮中显着下调。我们假设结膜上皮中 EP3 的下调与 SJS/TEN 和 OCP 的发病机制和病理学之间存在关联，并提出这些疾病的病理学中的共同机制。结膜上皮EP3蛋白表达的检查可能有助于各种眼表疾病的鉴别诊断。
In our earlier genome-wide association study on Stevens-Johnson Syndrome (SJS) and its severe variant, toxic epidermal necrolysis (TEN), we found that in Japanese patients with these severe ocular surface complications there was an association with prostaglandin E receptor 3 (EP3) gene (PTGER3) polymorphisms. We also reported that EP3 is dominantly expressed in the ocular surface-, especially the conjunctival epithelium, and suggested that EP3 in the conjunctival epithelium may down-regulate ocular surface inflammation. In the current study we investigated the expression of EP3 protein in the conjunctiva of patients with various ocular surface diseases such as SJS/TEN, chemical eye burns, Mooren’s ulcers, and ocular cicatricial pemphigoid (OCP). Conjunctival tissues were obtained from patients undergoing surgical reconstruction of the ocular surface due to SJS/TEN, chemical eye burns, and OCP, and from patients with Mooren's ulcers treated by resection of the inflammatory conjunctiva. The controls were nearly normal human conjunctival tissues acquired at surgery for conjunctivochalasis. We performed immunohistological analysis of the EP3 protein and evaluated the immunohistological staining of EP3 protein in the conjunctival epithelium of patients with ocular surface diseases. EP3 was expressed in the conjunctival epithelium of patients with chemical eye burns and Mooren’s ulcer and in normal human conjunctival epithelium. However, it was markedly down-regulated in the conjunctival epithelium of SJS/TEN and OCP patients. We posit an association between the down-regulation of EP3 in conjunctival epithelium and the pathogenesis and pathology of SJS/TEN and OCP, and suggest a common mechanism(s) in the pathology of these diseases. The examination of EP3 protein expression in conjunctival epithelium may aid in the differential diagnosis of various ocular surface diseases.
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影响因子：
4.1
作者：
Ueta, Mayumi;Matsuoka, Toshiyuki;Kinoshita, Shigeru
通讯作者：
Kinoshita, Shigeru
影响因子：
--
作者：
Matsuoka T;Narumiya S
通讯作者：
Narumiya S
影响因子：
14.2
作者：
Ueta, Mayumi;Matsuoka, Toshiyuki;Kinoshita, Shigeru
通讯作者：
Kinoshita, Shigeru
影响因子：
14.2
作者：
Honda, Tetsuya;Matsuoka, Toshiyuki;Narumiya, Shuh
通讯作者：
Narumiya, Shuh
影响因子：
14.2
作者：
Ueta, Mayumi;Sotozono, Chie;Kinoshita, Shigeru
通讯作者：
Kinoshita, Shigeru