Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering
Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering
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DOI：
10.1016/j.biomaterials.2005.05.043
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发表时间：
2005-12-01
期刊：
影响因子：
14
通讯作者：
Ghanem, A
中科院分区：
文献类型：
--
作者：
Adekogbe, I;Ghanem, A
壳聚糖是甲壳素的脱乙酰化衍生物，是一种很有前途的皮肤组织工程支架材料。它具有生物相容性和生物降解性，降解产物可吸收。然而，壳聚糖的快速降解和其低机械强度是可能限制其使用的问题。本研究以脱乙酰度（DDA）为80%、90%和100%的壳聚糖为材料，用3-3，二硫代双丙亚胺酸二甲酯（DTBP）交联，并与未交联的支架进行比较。100%DDA壳聚糖制成的支架的拉伸强度显著高于80%和90%DDA壳聚糖制成的支架。用DTBP交联支架增加拉伸强度。与DTBP交联的水蒸气透过率（WVTR）或吸水率没有显着的影响，但对样品的孔径和孔隙率有显着的影响。所有样品显示出适合皮肤组织工程的WVTR和孔径分布;然而，吸水率和孔隙率低于皮肤组织工程的最佳值。DTBP和戊二醛（GTA）交联的支架降解速率降低，而100%DDA壳聚糖交联和未交联的支架降解24 d后生物降解速率无明显变化。(c)2005爱思唯尔有限公司保留所有权利。
Chitosan, the deacetylated derivative of chitin, is a promising scaffold material for skin tissue engineering applications. It is biocompatible and biodegradable, and the degradation products are resorbable. However, the rapid degradation of chitosan and its low mechanical strength are concerns that may limit its use. In this study, chitosan with 80%, 90% and 100% degree of deacetylation (DDA) was crosslinked with dimethyl 3-3, dithio bis' propionimidate (DTBP) and compared to uncrosslinked scaffolds. The scaffolds were characterized with respect to important tissue engineering properties.The tensile strength of scaffolds made from 100% DDA chitosan was significantly higher than for scaffolds made from 80% and 90% DDA chitosan. Crosslinking of scaffolds with DTBP increased the tensile strength. Crosslinking with DTBP had no significant effect on water vapour transmission rate (WVTR) or water absorption but had significant effect on the pore size and porosity of the samples. All samples showed a WVTR and pore size distribution suitable for skin tissue engineering; however, the water absorption and porosity were lower than the optimal values for skin tissue engineering. The biodegradation rate of scaffolds crosslinked with DTBP and glutaraldehyde (GTA) were reduced while no significant effect was observed in biodegradation of the samples made from 100% DDA chitosan whether crosslinked or uncrosslinked after 24 days of degradation. (c) 2005 Elsevier Ltd. All rights reserved.