Robust methods to create ex vivo minimum deformation atlases for brain mapping
Robust methods to create ex vivo minimum deformation atlases for brain mapping
复制标题
DOI：
10.1016/j.ymeth.2015.01.005
复制
发表时间：
2015-02-01
期刊：
影响因子：
4.8
通讯作者：
Ullmann, Jeremy F. P.
中科院分区：
文献类型：
--
作者：
Janke, Andrew L.;Ullmann, Jeremy F. P.
高度详细的平均结构的离体3D图谱对神经科学及其当前推动理解大脑的全球微观结构至关重要。多个单层组织学切片不再能够提供足够的切片间显微结构细节，并且缺乏平面外分辨率。已经出现了两种离体方法，可以创建这样详细的模型。高场微MRI加上造影剂已经允许完整的全脑微结构成像的各向同性分辨率为15 μ m.Blockface成像类似地发展到一个点，现在它是可能的图像在一个严格的方式与面外分辨率为10 μ m的整个大脑。尽管作为该过程的一部分的组织的破坏，但其允许无切割伪影的重建模型。这两种方法都被用来创建最小变形图集，代表各自的人口。MDA图谱让我们前所未有的洞察到的共性和差异，在特定类群的皮质结构的微观结构。在本文中，我们提供了一个概述，如何创建这样的MDA模型从体外数据。(C)2015 Elsevier Inc. All rights reserved.
Highly detailed ex vivo 3D atlases of average structure are of critical importance to neuroscience and its current push to understanding the global microstructure of the brain. Multiple single slice histology sections can no longer provide sufficient detail of inter-slice microstructure and lack out of plane resolution. Two ex vivo methods have emerged that can create such detailed models. High-field micro MRI with the addition of contrast media has allowed intact whole brain microstructure imaging with an isotropic resolution of 15 mu m in mouse.Blockface imaging has similarly evolved to a point where it is now possible to image an entire brain in a rigorous fashion with an out of plane resolution of 10 mu m. Despite the destruction of the tissue as part of this process it allows a reconstructed model that is free from cutting artifacts. Both of these methods have been utilised to create minimum deformation atlases that are representative of the respective populations. The MDA atlases allow us unprecedented insight into the commonality and differences in microstructure in cortical structures in specific taxa. In this paper we provide an overview of how to create such MDA models from ex vivo data. (C) 2015 Elsevier Inc. All rights reserved.