Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium
Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium
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DOI：
10.1007/s00294-010-0309-z
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发表时间：
2010-10-01
期刊：
影响因子：
2.5
通讯作者：
Irie, Toshikazu
中科院分区：
文献类型：
--
作者：
Sakamoto, Takaiku;Kitaura, Hironori;Irie, Toshikazu
我们研究了钙调素（CaM）抑制剂W-7对黄孢原毛平革菌木质素过氧化物酶（LiP）和锰过氧化物酶（MNP）基因表达的影响，以考虑CaM基因的作用，CaM基因在我们先前的转录组学分析中与LiP和MNP的总活性平行上调。向真菌培养物中加入100 μ M W-7抑制了LiP和MnP的总活性，而加入100 μ M W-5（W-7的对照药物）则保留了约一半的活性，表明W-7的作用可归因于CaM抑制。实时荧光定量逆转录聚合酶链反应（RT-PCR）分析表明，W-7在转录水平上显著抑制了全基因组数据预测的大部分lip和mnp同工酶基因（P <0.05）。这些结果表明，CaM在转录水平上对LiP和MNP同工酶基因的表达具有重要作用。
We investigated the effects of a calmodulin (CaM) inhibitor, W-7, on the expression of lignin peroxidase (LiP) and manganese peroxidase (MnP) genes in Phanerochaete chrysosporium to consider the role of cam gene, which was upregulated in parallel with the total activities of LiP and MnP in our previous transcriptomic analysis. The addition of 100 mu M W-7 to the fungal cultures repressed the total activities of LiP and MnP, whereas the addition of 100 mu M W-5, which is a control drug of W-7, retained approximately half of them, indicating that the effect of W-7 was attributable to CaM inhibition. Real-time reverse transcription polymerase chain reaction analysis revealed that most of lip and mnp isozyme genes predicted from whole-genome data were significantly inhibited by W-7 at the transcription level (P a parts per thousand currency sign 0.05). These results suggest that CaM has an important role for the expression of isozyme genes of LiP and MnP at the transcription level.