Dissection of two quantitative trait loci with pleiotropic effects on plant height and spike length linked in coupling phase on the short arm of chromosome 2D of common wheat (Triticum aestivum L.)
Dissection of two quantitative trait loci with pleiotropic effects on plant height and spike length linked in coupling phase on the short arm of chromosome 2D of common wheat (Triticum aestivum L.)
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DOI：
10.1007/s00122-019-03420-2
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发表时间：
2019-06-01
影响因子：
5.4
通讯作者：
Ni, Zhongfu
中科院分区：
文献类型：
--
作者：
Chai, Lingling;Chen, Zhaoyan;Ni, Zhongfu
对位于第2DS染色体上的两个与株高和穗长连锁的具有多效效应的QTL进行了剖析，并为每个QTL建立了诊断标记。摘要株高是与植株结构和产量潜力相关的重要性状，剖析其潜在的遗传基础有助于提高小麦设计育种的效率。这里，两个数量性状基因座（QTL）在2D染色体短臂上的偶联期连锁，对PHT和穗长QPht/Sl具有多效性效应。cau-2D.1和QPht/Sl. cau-2D.2进行了分离和表征。QPht/Sl. cau-2D.1是位于SNP标记BS00022234_51和BobWhite_rep_c63957_1472之间的一个新QTL。QPht/Sl. cau-2D.2定位在与Rht 8位于同一基因组区间的SSR标记SSR-2062和Xgwm 484之间。此外，与每个QTL紧密连锁的诊断标记被开发用于利用不同的小麦种质进行单倍型分析。QPht/S1的身高降低等位基因的频率。cau-2D.1比QPht/S1低得多。cau-2D.2，表明该新QTL可能是一个有吸引力的遗传改良目标。与Rht 8的先前研究一致，在QPht/Sl的NIL之间观察到细胞长度的显著差异。cau-2D.2.相比之下，QPht/Sl的NIL之间的细胞长度没有差异。cau-2D.1，表明这两个QTL的潜在分子机制可能不同。总的来说，这些数据提供了一个新的QTL解剖的例子，和开发的诊断标记将是有用的标记辅助的QTL/S1的分离。cau-2D.1和/或QPht/S1。cau-2D.2与其他基因在小麦育种中的应用。
Key message Two QTL with pleiotropic effects on plant height and spike length linked in coupling phase on chromosome 2DS were dissected, and diagnostic marker for each QTL was developed.Abstract Plant height (PHT) is a crucial trait related to plant architecture and yield potential, and dissection of its underlying genetic basis would help to improve the efficiency of designed breeding in wheat. Here, two quantitative trait loci (QTL) linked in coupling phase on the short arm of chromosome 2D with pleiotropic effects on PHT and spike length, QPht/Sl. cau-2D.1 and QPht/Sl. cau-2D.2, were separated and characterized. QPht/Sl. cau-2D.1 is a novel QTL located between SNP makers BS00022234_51 and BobWhite_rep_c63957_1472. QPht/Sl. cau-2D.2 is mapped between two SSR markers, SSR-2062 and Xgwm484, which are located on the same genomic interval as Rht8. Moreover, the diagnostic marker tightly linked with each QTL was developed for the haplotype analysis using diverse panels of wheat accessions. The frequency of the height-reduced allele of QPht/Sl. cau-2D.1 is much lower than that of QPht/Sl. cau-2D.2, suggesting that this novel QTL may be an attractive target for genetic improvement. Consistent with a previous study of Rht8, a significant difference in cell length was observed between the NILs of QPht/Sl. cau-2D.2. By contrast, there was no difference in cell length between NILs of QPht/Sl. cau-2D.1, indicating that the underlying molecular mechanism for these two QTL may be different. Collectively, these data provide a new example of QTL dissection, and the developed diagnostic markers will be useful in marker-assisted pyramiding of QPht/Sl. cau-2D.1 and/or QPht/Sl. cau-2D.2 with the other genes in wheat breeding.