Costimulatory Molecule CD28 Participates in the Process of Embryo Implantation in Mice
Costimulatory Molecule CD28 Participates in the Process of Embryo Implantation in Mice
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共刺激分子CD28参与小鼠胚胎着床过程
DOI：
10.1177/1933719113512537
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发表时间：
2014-06-01
影响因子：
2.9
通讯作者：
Wang, Yingxiong
中科院分区：
文献类型：
--
作者：
Liu, Shangjing;He, Junlin;Wang, Yingxiong
胚胎植入是一个复杂的过程，需要有发育能力的囊胚和接受子宫之间的相互作用。大量文献表明T细胞参与了这一过程。由T细胞受体/CD 3复合物介导的第一信号和由共刺激分子传递的第二信号对于T细胞分化为效应细胞是必需的。CD 28是一种重要的共刺激分子，其在小鼠妊娠早期的表达和功能尚不清楚。在本研究中，我们研究了CD 28的表达模式，在早期妊娠和假孕小鼠子宫实时定量聚合酶链反应，免疫印迹，原位杂交和免疫组织化学（IHC）。我们发现，注射的子宫角与CD 28反义寡脱氧核苷酸导致植入位点的数量减少。免疫组化检测CD 3蛋白表达模式与CD 28相似。提示CD 28参与小鼠胚胎着床过程，可能通过传递第二共刺激信号发挥作用。
Embryo implantation is a complex process requiring reciprocal interactions between implantation-competent blastocysts and receptive uteri. Accumulating literatures have indicated that T cells are involved in this process. The first signal mediated by T-cell receptor/CD3 complex and the second signal delivered by costimulatory molecules are essential for the differentiation of T cell into an effector cell. Expression and function of CD28, an important costimulatory molecule, during early pregnancy in mice is still unclear. In the present study, we investigated the expression pattern of CD28 in mouse uterus during early pregnancy and pseudopregnancy by real-time quantitative polymerase chain reaction, Western blotting, in situ hybridization, and immunohistochemistry (IHC). We found that injection of the uterine horn with CD28 antisense oligodeoxynucleotides leads to a decreased number of implantation sites. The expression pattern of CD3 protein examined by IHC is similar to that of CD28. These findings suggest that CD28 participates in the process of embryo implantation in mice, which might play its role through delivering the second costimulatory signal.