Beyond editing, CRISPR/Cas9 for protein localization: an educational primer for use with “A dCas9-based system identifies a central role for Ctf19 in kinetochore-derived suppression of meiotic recombination”
Beyond editing, CRISPR/Cas9 for protein localization: an educational primer for use with “A dCas9-based system identifies a central role for Ctf19 in kinetochore-derived suppression of meiotic recombination”
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除了编辑之外，用于蛋白质定位的 CRISPR/Cas9：与“基于 dCas9 的系统确定了 Ctf19 在动粒衍生的减数分裂重组抑制中的核心作用”一起使用的教育引物
DOI：
10.1093/genetics/iyac109
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发表时间：
2022
期刊：
影响因子：
3.3
通讯作者：
De Stasio, ed., E.
中科院分区：
文献类型：
--
作者：
McVey, Shelby L.;Olson, Mischa A.;Pawlowski, Wojciech P.;Nannas, Natalie J.;De Stasio, ed., E.
CRISPR/Cas9极大地改变了我们进行遗传研究的方式，为精确的序列编辑提供了工具。然而，CRISPR/Cas9的新应用已经出现，不涉及核酸酶活性。在随附的文章“基于dCas 9的系统确定了Ctf 19在着丝粒衍生的减数分裂重组抑制中的中心作用”中，Kuhlet等人利用催化死亡的Cas9将蛋白质定位在特定的基因组位置。作者试图了解着丝粒蛋白在抑制减数分裂重组中的作用，这是一种在着丝粒区域观察到的现象。通过利用CRISPR/Cas9结合特定基因组序列的能力，Kuhlet等人将单个动粒蛋白定位于高度减数分裂重组的区域，并评估了它们在抑制中的作用。这篇入门文章为本科生提供了关于染色体、减数分裂、重组和CRISPR/Cas9的背景信息，以支持他们阅读Kuhlet等人的研究。本入门旨在帮助学生和教师导航研究的实验设计，解释结果，并欣赏减数分裂重组和CRISPR/Cas9的更广泛范围。为便于讨论研究报告，提出了一些问题。
CRISPR/Cas9 has dramatically changed how we conduct genetic research, providing a tool for precise sequence editing. However, new applications of CRISPR/Cas9 have emerged that do not involve nuclease activity. In the accompanying article “A dCas9-based system identifies a central role for Ctf19 in kinetochore-derived suppression of meiotic recombination,” Kuhlet al.utilize a catalytically dead Cas9 to localize proteins at specific genomic locations. The authors seek to understand the role of kinetochore proteins in the suppression of meiotic recombination, a phenomenon that has been observed in centromere regions. By harnessing the power of CRISPR/Cas9 to bind specific genomic sequences, Kuhlet al.localized individual kinetochore proteins to areas of high meiotic recombination and assessed their role in suppression. This primer article provides undergraduate students with background information on chromosomes, meiosis, recombination and CRISPR/Cas9 to support their reading of the Kuhlet al.study. This primer is intended to help students and instructors navigate the study’s experimental design, interpret the results, and appreciate the broader scope of meiotic recombination and CRISPR/Cas9. Questions are included to facilitate discussion of the study.