Mild hyperthermia enhances the expression and induces oscillations in the Dicer protein.
Mild hyperthermia enhances the expression and induces oscillations in the Dicer protein.
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DOI：
10.3109/02656736.2012.753471
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Tomasi TB
中科院分区：
文献类型：
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作者：
Oshlag JZ;Devasthanam AS;Tomasi TB
研究39.5°C的轻度热应激是否改变了几种细胞类型中的Dicer蛋白和miRNA表达模式。在37°C至39.5°C的温度下，在9小时的过程中培养多种人类和小鼠细胞类型。通过TaqMan RT-qPCR测定定量Dicer mRNA水平和microRNA，并通过蛋白质印迹定量Dicer蛋白。通过RT-qPCR，在Dicer mRNA不存在显著变化的情况下，响应于39.5°C的热应激，Dicer蛋白在Western分析中显著升高。Dicer表达的热诱导调节主要发生在转录后，并且Dicer蛋白的表达水平增加，并且通常响应于多种小鼠和人细胞中的发热范围的高温而振荡。我们的研究表明Dicer和microRNA在对轻度热应激的反应中具有潜在的作用。对应激诱导的Dicer蛋白和microRNA振荡机制的进一步研究将是有意义的。
To investigate whether mild heat stress at 39.5°C altered Dicer protein and miRNA expression patterns in several cell types. Multiple human and mouse cell types were cultured during the course of 9 h at temperatures from 37°C to 39.5°C. Dicer mRNA levels and microRNAs were quantified by TaqMan RT-qPCR assays and Dicer protein by western blotting. Dicer protein was substantially elevated on western analysis in response to heat stress at 39.5°C in the absence of significant changes in Dicer mRNA by RT-qPCR. Heat-induced regulation of Dicer expression occurs primarily post-transcriptionally, and the expression levels of Dicer protein are increased and often oscillate in response to fever-range hyperthermia in multiple mouse and human cells. Our studies suggest a potential role for Dicer and microRNAs in the response to mild thermal stress. Additional studies on the mechanisms involved in the stress-induced oscillations of Dicer protein and microRNAs will be of interest.