Molecular mapping of human hepatocellular carcinoma provides deeper biological insight from genomic data
Molecular mapping of human hepatocellular carcinoma provides deeper biological insight from genomic data
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DOI：
10.1016/j.ejca.2008.02.019
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发表时间：
2008-04-01
影响因子：
8.4
通讯作者：
Monden, Morito
中科院分区：
文献类型：
--
作者：
Kittaka, Nobuyoshi;Takemasa, Ichiro;Monden, Morito
人类癌症的 DNA 微阵列分析已导致全球基因谱的大量积累。然而，提取和理解癌症进展的潜在生物学仍然是一个重大挑战。这项研究应用了一种新颖的综合计算和分析方法来应对人类肝细胞癌 (HCC) 的这一挑战，旨在识别潜在的分子标记或新的治疗靶点。我们通过人类 30 K DNA 微阵列分析了 100 个 HCC 组织样本。将基因表达数据上传到网络分析工具中，并以图形方式显示生物网络。我们发现了几个含有上调基因集中的激活“热点”区域。几个“热点”区域揭示了整合素和 Akt/NF-kappa B 信号传导。我们确定了与这些信号通路相关的关键成员，包括骨桥蛋白 (SPP1)、磷脂酰肌醇蛋白聚糖-3 (GPC3)、膜联蛋白 2 (ANXA2)、S100A10 和波形蛋白 (VIM)。我们的综合方法应该显着增强微阵列数据在识别人类癌症新的潜在靶标方面的能力。 (c) 2008 Elsevier Ltd. 保留所有权利。
DNA microarray analysis of human cancer has resulted in considerable accumulation of global gene profiles. However, extraction and understanding the underlying biology of cancer progression remains a significant challenge. This study applied a novel integrative computational and analytical approach to this challenge in human hepatocellular carcinoma (HCC) with the aim of identifying potential molecular markers or novel therapeutic targets. We analysed 100 HCC tissue samples by human 30 K DNA microarray. The gene expression data were uploaded into the network analysis tool, and the biological networks were displayed graphically. We identified several activated 'hotspot' regions harbouring a concentration of upregulated genes. Several 'hotspot' regions revealed integrin and Akt/NF-kappa B signalling. We identified key members linked to these signalling pathways including osteopontin (SPP1), glypican-3 (GPC3), annexin 2 (ANXA2), S100A10 and vimentin (VIM). Our integrative approach should significantly enhance the power of microarray data in identifying novel potential targets in human cancer. (c) 2008 Elsevier Ltd. All rights reserved.