Efficient disulfide bond formation in virus-like particles
Efficient disulfide bond formation in virus-like particles
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DOI：
10.1016/j.jbiotec.2011.04.011
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发表时间：
2011-07-20
影响因子：
4.1
通讯作者：
Swartz, James R.
中科院分区：
文献类型：
--
作者：
Bundy, Bradley C.;Swartz, James R.
病毒样颗粒(VLP)由病毒的外壳组成，但没有基因组。与病毒相似，VLP是单分散的纳米胶囊，具有已知的形态，保持高度的对称性，并且可以被设计成包裹所需的货物。VLP在疫苗接种、药物/基因传递、成像、传感和材料科学应用方面具有重要意义。在这里，我们展示了通过在生产和组装VLP期间或之后直接控制氧化还原电位来控制VLP中二硫键形成的能力。采用了开放的无细胞蛋白质合成环境，据报道，这种环境可以产生与传统体内技术相当或更高的VLP。二硫键形成的最佳条件依赖于VLP，并且在这种键的形成中观察到了协同效应。(C)2011爱思唯尔B.V.保留所有权利。
Virus-like particles (VLPs) consist of a virus's outer shell but without the genome. Similar to the virus, VLPs are monodisperse nano-capsules which have a known morphology, maintain a high degree of symmetry, and can be engineered to encapsidate the desired cargo. VLPs are of great interest for vaccination, drug/gene delivery, imaging, sensing, and material science applications. Here we demonstrate the ability to control the disulfide bond formation in VLPs by directly controlling the redox potential during or after production and assembly of VLPs. The open cell-free protein synthesis environment, which has been reported to produce VLPs at yields comparable or greater than traditional in vivo technologies, was employed. Optimal conditions for disulfide bond formation were found to be VLP dependent, and a cooperative effect in the formation of such bonds was observed. (C) 2011 Elsevier B. V. All rights reserved.