CATECHOLAMINE EFFECTS ON TESTICULAR TESTOSTERONE PRODUCTION IN THE GONADALLY ACTIVE AND THE GONADALLY REGRESSED ADULT GOLDEN-HAMSTER
CATECHOLAMINE EFFECTS ON TESTICULAR TESTOSTERONE PRODUCTION IN THE GONADALLY ACTIVE AND THE GONADALLY REGRESSED ADULT GOLDEN-HAMSTER
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DOI：
10.1095/biolreprod40.4.752
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发表时间：
1989-04-01
影响因子：
3.6
通讯作者：
STEGER, RW
中科院分区：
文献类型：
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作者：
MAYERHOFER, A;BARTKE, A;STEGER, RW
一些证据支持睾丸神经支配和外周儿茶酚胺在男性性腺功能控制中的作用，特别是在青春期之前。因此，比较儿茶酚胺对季节性繁殖物种性腺活跃和静止期雄激素产生的影响是很有意义的。我们研究了肾上腺素(EPI)、去甲肾上腺素(NE)、β-肾上腺素能激动剂异丙肾上腺素(ISO)和α-肾上腺素能激动剂苯肾上腺素(Phe)对短光周期(SD)所致性腺退行性变(SD)和维持长光周期(LD)性腺活动仓鼠睾丸睾酮(T)产生的直接影响。用这些儿茶酚胺(10~(-5)~10~(-9)M)、人绒毛膜促性腺激素(hCG；3.1mIU/ml)、β受体拮抗剂普萘洛尔(10~(-5)M)和α-1受体拮抗剂哌唑嗪(10~(-5)M)的不同组合孵育6h。EPI、NE和Phe在10~(-5)M时可增强hCG对T的刺激作用，而ISO无此作用。与此形成鲜明对比的是，EPI(10-8-10-5M)、NE(10-6-10-5M)和Phe(10-6-10-5M)以剂量依赖的方式刺激SD动物退化的睾丸产生T，但不受ISO的影响。这些兴奋作用可被哌唑嗪阻止，但不能被普萘洛尔阻止。此外，10-5M的EPI、NE和Phe可增强hCG对T产生的刺激作用。我们的结论是，成年金黄地鼠从性腺活动到静止的季节性转变伴随着睾丸类固醇合成对儿茶酚胺的反应性的显著增加，儿茶酚胺通过α-1肾上腺素受体作用于该物种，儿茶酚胺可以调节间质细胞对促性腺激素的反应。这些发现可能与SD动物睾丸中α-1受体的上调有关，并提示儿茶酚胺可能参与了生理性抑制促性腺激素释放和应激期间睾丸的调节。
Several lines of evidence support a role of testicular innervation and peripheral catecholamines in the control of male gonadal function, particularly before puberty. It was therefore of interest to compare the effects of catecholamines on androgen production during the periods of gonadal activity and quiescence in a seasonally breeding species. We have examined direct effects of epinephrine (EPI), norepinephrine (NE), the beta-adrenergic agonist isoproterenol (ISO), and the alpha-adrenergic agonist phenylephrine (PHE) on testicular testosterone (T) production in hamsters with gonadal regression induced by 12 wk exposure to short photoperiod (SD) and in gonadally active hamsters maintained in long photoperiod (LD). Fragments of decapsulated testes were incubated with various combinations of these catecholamines (10-5-10-9 M), human chorionic gonadotropin (hCG; 3.1 mIU/ml), the beta-receptor antagonist propranolol (10-5 M) and the alpha-1-receptor antagonist prazosin (10-5 M), for 6 h. In the incubations of testes from LD hamsters, the accumulation of T in the medium was stimulated by hCG but not affected by either catecholamine. However, EPI, NE, and PHE at 10-5 M, but not ISO, augmented the stimulation of T by hCG. In sharp contrast to these findings, T production by the regressed testes of SD animals was stimulated by EPI (at 10-8-10-5 M), NE (at 10-6-10-5 M), and PHE (at 10-6-10-5 M) in a dose-related manner, but unaffected by ISO. These stimulatory effects were prevented by prazosin, but not by propranolol. Moreover, 10-5 M of EPI, NE, and PHE augmented the stimulatory effect of hCG on T production. We conclude that the seasonal transition from gonadal activity to quiescence in the adult golden hamster is accompanied by a major increase in the responsiveness of testicular steroidogenesis to catecholamines acting via the alpha-1-adrenoreceptor and that catecholamines can modulate Leydig cell response to gonadotropins in this species. These findings could be related to up-regulation of the alpha-1-receptor in the testis of the SD animal and suggest that catecholamines may be involved in the regulation of the testis during physiological suppression of gonadotropin release and during stress.