A simplified multidimensional approach for analysis of complex biological samples:: on-line LC-CE-MS
A simplified multidimensional approach for analysis of complex biological samples:: on-line LC-CE-MS
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DOI：
10.1039/b601660j
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发表时间：
2006-01-01
期刊：
影响因子：
4.2
通讯作者：
Bergquist, Jonas
中科院分区：
文献类型：
--
作者：
Bergstroem, Sara K.;Dahlin, Andreas P.;Bergquist, Jonas
关于蛋白质表达、疾病生物标志物或替代标志物和遗传病症的信息如今可以通过结合质谱（MS）检测的液体分离从复杂生物样品的分析中获得。本文介绍了快速多维分离的在线液相色谱（LC）和毛细管电泳（CE），然后电喷雾电离（ESI）傅立叶变换离子回旋共振（FTICR）MS检测。该检测器提供了被检测离子的高分辨率、ppm级的质量准确度和高灵敏度。该系统的最大挑战在于开发一种新的接口，用于LC到CE的在线耦合。在聚二甲基硅氧烷中开发的界面提供了进样重复性的RSD：
Information on protein expression, disease biomarkers or surrogate markers and genetic disorders can nowadays be achieved from analysis of complex biological samples by liquid separation coupled to mass spectrometric (MS) detection. This paper describes fast multidimensional separation by on-line liquid chromatography (LC) and capillary electrophoresis (CE), followed by electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) MS detection. This detector provides ultrahigh resolution of the detected ions, mass accuracy at the ppm-level and high sensitivity. Most of the challenge of this system lies in the development of a new interface for the on-line coupling of LC to CE. The interface developed in poly(dimethylsiloxane) provides a RSD for injection repeatability of