Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells
Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells
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DOI：
10.1007/s00251-006-0136-z
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发表时间：
2006-09-01
期刊：
影响因子：
3.2
通讯作者：
Baldwin, Cynthia L.
中科院分区：
文献类型：
--
作者：
Herzig, Carolyn T. A.;Blumerman, Seth L.;Baldwin, Cynthia L.
为了了解反刍动物γ δ T细胞的生物学特性，有必要对γ δ T细胞受体基因的多样性和表达有一个全面的了解。本研究通过RT-PCR鉴定了牛t细胞受体δ (TRD)可变基因的3个新亚群，并对其与其他哺乳动物的TRDV基因进行了测序和同源性分析。本研究中描述的先前未确定的TRDV亚群基因包括牛的TRDV2、TRDV3和TRDV4同源基因，并相应地命名。TRDV2亚群有两个基因（TRDV2-1和TRDV2-2），而我们发现先前鉴定的TRDV1至少有8个基因对应于单独的基因组序列。牛和羊之间特定基因亚群的核苷酸和氨基酸序列相同度超过87%，但同一物种内TRDV亚群之间的同源性要低得多，牛TRDV4与其他三个牛TRDV基因亚群的同源性< 45%。对循环牛γ δ T细胞的分析显示，所有四个TRDV亚组的基因都与TRDJ1、TRDJ3和TRDC联合表达，尽管TRDV4的表达最少，并且都表现出不同的CDR3连接长度。最后，TRDV1、TRDV2和TRDV3亚群中的一些基因与含有traj的TRAV重组，表明具有双重用途。
To understand the biology of gamma delta T cells in ruminants, it is necessary to have a comprehensive picture of gamma delta T-cell receptor gene diversity and expression. In this study, three new subgroups of bovine T-cell receptor delta (TRD) variable genes were identified by RT-PCR and sequencing and homology with TRDV genes from other mammals determined. Previously unidentified TRDV subgroup genes described in this study include the bovine homologues of ovine TRDV2, TRDV3, and TRDV4 which were named accordingly. TRDV2 subgroup has two genes (TRDV2-1 and TRDV2-2) while we found the previously identified TRDV1 has at least eight genes corresponding to separate genomic sequences. Nucleotide and amino acid sequences for particular gene subgroups between cattle and sheep were more than 87% identical but identities among TRDV subgroups within a species were much less, with bovine TRDV4 having < 45% identity to the other three bovine TRDV gene subgroups. Analysis of circulating bovine gamma delta T cells revealed that genes from all four TRDV subgroups were expressed in combination with TRDJ1, TRDJ3, and TRDC, although TRDV4 was the least represented, and all displayed a variety of CDR3 junctional lengths. Finally, some genes within the TRDV1, TRDV2, and TRDV3 subgroups recombined with TRAV incorporating TRAJs, suggesting dual use.