Cross-linking of Qa-2 protein, the Ped gene product, increases the cleavage rate of C57BL/6 preimplantation mouse embryos.
Cross-linking of Qa-2 protein, the Ped gene product, increases the cleavage rate of C57BL/6 preimplantation mouse embryos.
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Ped 基因产物 Qa-2 蛋白的交联可提高 C57BL/6 植入前小鼠胚胎的卵裂率。
DOI：
10.1093/molehr/6.6.517
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发表时间：
2000
影响因子：
4
通讯作者：
Warner,CM
中科院分区：
文献类型：
--
作者：
McElhinny,AS;Warner,CM
Qa-2蛋白是一种与糖基磷脂酰肌醇（GPI）相关的主要组织相容性复合体（MHC） Ib类分子，存在于小鼠t细胞和着床前胚胎表面，是着床前胚胎发育（Ped）基因的产物。pedgene调节早期胚胎发育和随后胚胎存活的速率。在共刺激信号存在的情况下，用抗qa -2单克隆抗体（mAb）处理并与二抗交联的t细胞增殖增加。本研究的目的是确定Qa-2交联是否同样影响着床前胚胎。我们将Qa-2蛋白交联在C57BL/6的2细胞和8细胞胚胎表面，在4/5- phorpol -12-肉豆酸酯-13-乙酸酯（PMA）存在的情况下，评估胚胎进入囊胚期的百分比，从透明带孵化的百分比，[3h -胸腺嘧啶]与DNA结合的百分比，以及每个胚胎的细胞总数作为胚胎分裂率的指标。交联Qa-2后48 h， 2细胞胚和8细胞胚的卵裂率均高于对照胚（P< 0.05）。我们的研究结果表明，Qa-2蛋白的交联机制可能是该蛋白调节着床前小鼠胚胎发育速率的一种方式。
The Qa-2 protein, a glycosylphosphatidylinositol (GPI)-linked major histocompatibility complex (MHC) Class Ib molecule found on the surface of mouse T-cells and preimplantation embryos, is the product of the preimplantation embryo development(Ped)gene. ThePedgene regulates the rate of early embryonic development and subsequent embryo survival. T-cells treated with anti-Qa-2 monoclonal antibody (mAb) and cross-linked with a secondary antibody, in the presence of a co-stimulatory signal, undergo increased proliferation. The purpose of this study was to determine whether cross-linking of Qa-2 similarly affects preimplantation embryos. We cross-linked Qa-2 protein on the surface of C57BL/6 2-cell and 8-cell embryos, in the presence of 4/5-phorbol-12-myristate-13-acetate (PMA), and assessed the percentage of embryos reaching the blastocyst stage, the percentage hatching from the zona pellucida, [3H-thymidine] incorporation into DNA, and the total number of cells per embryo as measures of embryonic cleavage rate. Both 2-cell and 8-cell embryos increased their cleavage rates 48 h after cross-linking of Qa-2, compared with control embryos (P< 0.05). Our results indicate that a Qa-2 protein cross-linking mechanism may be one way by which this protein regulates the rate of preimplantation mouse embryo development.