Hsa_circ_0005379 regulates malignant behavior of oral squamous cell carcinoma through the EGFR pathway (Retracted Article)
Hsa_circ_0005379 regulates malignant behavior of oral squamous cell carcinoma through the EGFR pathway (Retracted Article)
复制标题
Hsa_circ_0005379通过EGFR通路调控口腔鳞癌恶性行为
DOI：
10.1186/s12885-019-5593-5
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发表时间：
2019-04-29
期刊：
影响因子：
3.8
通讯作者：
Yang, Hongyu
中科院分区：
文献类型：
--
作者：
Su, Wen;Wang, Yufan;Yang, Hongyu
口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）是一种世界性的口腔颌面部恶性肿瘤。越来越多的证据表明，环状RNA（circRNA）在调节肿瘤的发展中起着至关重要的作用。然而，circRNA在人类OSCC中的作用机制在很大程度上仍然未知。MethodsBy使用高通量转录组测序技术，我们进行了全面的研究circRNA在人类OSCC。通过qRT-PCR、Transwell测定、流式细胞术和蛋白质印迹分析监测circRNA hsa_circ_0005379对OSCC组织和细胞系的作用。异种移植小鼠模型被用来评估肿瘤生长和动物survival.ResultsWe发现，circRNA hsa_circ_0005379的表达是显着较低的OSCC组织相比，配对的非癌匹配的组织，并与肿瘤的大小和分化。hsa_circ_0005379过表达可有效抑制口腔鳞癌细胞的迁移、侵袭和增殖，并抑制裸鼠口腔鳞癌的生长。机制研究表明，hsa_circ_0005379可能参与表皮生长因子受体（EGFR）途径的调节。此外，我们发现，hsa_circ_0005379的高表达可以显着提高敏感性的OSCC的西妥昔单抗drug.ConclusionsOur研究结果提供证据表明，hsa_circ_0005379调节OSCC恶性肿瘤，可能是一个新的治疗目标，为OSCC的治疗。
BackgroundOral squamous cell carcinoma (OSCC) is an oral and maxillofacial malignancy with a high incidence worldwide. Accumulating evidence indicates that circular RNAs (circRNAs) play a vital role in modulating tumor development. However, the mechanism of circRNA action in human OSCC remains largely unknown.MethodsBy using high-throughput transcriptome sequencing technology, we conducted a comprehensive study of circRNAs in human OSCC. The effect of circRNA hsa_circ_0005379 on OSCC tissues and cell lines was monitored by qRT-PCR, Transwell assay, flow cytometry, and western blot analysis. Xenograft mouse models were used to assess tumor growth and animal survival.ResultsWe found that circRNA hsa_circ_0005379 expression is significantly lower in OSCC tissue compared to paired non-cancerous matched tissue and is associated with tumor size and differentiation. Overexpression of hsa_circ_0005379 effectively inhibits migration, invasion, and proliferation of OSCC cells in vitro and suppresses OSCC growth in nude mice in vivo. Mechanistic studies revealed that hsa_circ_0005379 may be involved in the regulation of the epidermal growth factor receptor (EGFR) pathway. Furthermore, we found that high expression of hsa_circ_0005379 could significantly enhance the sensitivity of OSCC to the cetuximab drug.ConclusionsOur findings provide evidence that hsa_circ_0005379 regulates OSCC malignancy and may be a new therapeutic target for OSCC treatment.