Gene expression profiling of mucosal addressin cell adhesion molecule-1+ high endothelial venule cells (HEV) and identification of a leucine-rich HEV glycoprotein as a HEV marker
Gene expression profiling of mucosal addressin cell adhesion molecule-1+ high endothelial venule cells (HEV) and identification of a leucine-rich HEV glycoprotein as a HEV marker
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DOI：
10.4049/jimmunol.168.3.1050
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发表时间：
2002-02-01
影响因子：
4.4
通讯作者：
Miyasaka, M
中科院分区：
文献类型：
--
作者：
Saito, K;Tanaka, T;Miyasaka, M
高内皮微静脉（REV）细胞支持淋巴细胞从外周血迁移到次级淋巴组织。通过定量收集3 '-cDNA，利用粘膜地址素细胞粘附分子-1（+）肠系膜淋巴结HEV细胞的基因表达谱，我们鉴定了一种富含亮氨酸的蛋白质，命名为富含亮氨酸的HEV糖蛋白（LRHG），该蛋白质选择性地在这些细胞中表达。北方印迹分析显示，LRHG mRNA类似于1.3 kb，在淋巴结、肝脏和心脏中表达。原位杂交分析表明淋巴结中的mRNA表达严格限于HEV细胞，多克隆抗体的免疫荧光分析表明，LRHG蛋白主要定位于HEV细胞，并可能在一些淋巴。围绕着HEV的细胞。LRHG cDNA编码342个氨基酸的蛋白质，含有8个串联的富含亮氨酸的重复序列，每个重复序列24个氨基酸，与人富含亮氨酸的α（2）-糖蛋白具有高度同源性。类似于一些其他富含亮氨酸的重复蛋白家族成员，LRHG可以结合在HEV的基底层上表达的细胞外基质蛋白，如纤连蛋白、胶原IV和层粘连蛋白。此外，LRHG结合TGF-β。这些结果表明，LRHG可能是多功能的，因为它可以捕获TGF-β和/或其他相关的体液因子来调节细胞粘附局部，也可能参与HEV细胞粘附到周围的基底层。
High endothelial venule (REV) cells support lymphocyte migration from the peripheral blood into secondary lymphoid tissues. Using gene expression profiling of mucosal addressin cell adhesion molecule-1(+) mesenteric lymph node HEV cells by quantitative 3'-cDNA collection, we have identified a leucine-rich protein, named leucine-rich HEV glycoprotein (LRHG) that is selectively expressed in these cells. Northern blot analysis revealed that LRHG mRNA is similar to 1.3 kb and is expressed in lymph nodes, liver, and heart. In situ hybridization analysis demonstrated that the mRNA expression in lymph nodes is strictly restricted to the HEV cells, and immunofluorescence analysis with polyclonal Abs against LRHG indicated that the LRHG protein is localized mainly to HEV cells and possibly to some lymphoid. cells surrounding the HEVs. LRHG cDNA encodes a 342-aa protein containing 8 tandem leucine-rich repeats of 24 aa each and has high homology to human leucine-rich alpha(2)-glycoprotein. Similar to some other leucine-rich repeat protein family members, LRHG can bind extracellular matrix proteins that are expressed on the basal lamina of HEVs, such as fibronectin, collagen IV, and laminin. In addition, LRHG binds TGF-beta. These results suggest that LRHG is likely to be multifunctional in that it may capture TGF-beta and/or other related humoral factors to modulate cell adhesion locally and may also be involved in the adhesion of HEV cells to the surrounding basal lamina.