Aquimarina pacifica sp. nov., isolated from seawater.
Aquimarina pacifica sp. nov., isolated from seawater.
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DOI：
10.1099/ijs.0.062695-0
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发表时间：
2014-06
影响因子：
2.8
通讯作者：
Zenghu Zhang;Tong Yu;Tingting Xu;Xiaohua Zhang
中科院分区：
文献类型：
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作者：
Zenghu Zhang;Tong Yu;Tingting Xu;Xiaohua Zhang
在综合海洋钻探计划第 329 次探险期间，从南太平洋环流 (39° 19' S 139° 48' W) 的表层海水中分离出一种革兰氏染色阴性、杆状、无鞭毛、具有滑行运动能力的严格需氧细菌。在 2-4% (w/v) NaCl、pH 值下，最佳生长发生7-8 和 28-30°C。主要脂肪酸是 iso-C(15:0)、iso-C(17:0) 3-OH、iso-C(15:1)G、C(16:1)ω6c 和/或 C(16:1)ω7c 和 10-甲基 C(16:0) 和/或 iso-C(17:1)ω9c。菌株SW150(T)的极性脂质包含磷脂酰乙醇胺、三种未知极性脂质和一种未知氨基脂质。主要的呼吸醌是 MK-6。菌株SW150(T)的DNA G+C含量为33.8mol％。基于16S rRNA基因序列的系统发育分析表明，该新菌株与Aquimarina macrocephali JAMB N27(T)和Aquimarina muelleri KMM 6020(T)亲缘关系最近，序列相似度分别为97.8%和96.8%。菌株SW150(T)和A.macrocephali JAMB N27(T)之间的估计DNA-DNA杂交值为21.00±2.33%，菌株SW150(T)和Aquimarina megaterium XH134(T)之间的DNA-DNA杂交值为20.60±2.32%。根据多相分析，菌株 SW150(T) 代表了 Aquimarina 属的一个新种，其名称为 Aquimarina pacifica sp。十一月被提议。典型菌株为 SW150(T) ( = JCM 18214(T) = CGMCC 1.12180(T))。
A Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic bacterium with gliding motility, designated strain SW150(T), was isolated from surface seawater of the South Pacific Gyre (39° 19' S 139° 48' W) during the Integrated Ocean Drilling Program Expedition 329. Optimal growth occurred in the presence of 2-4% (w/v) NaCl, at pH 7-8 and at 28-30 °C. The dominant fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH, iso-C(15 : 1) G, C(16 : 1)ω6c and/or C(16 : 1)ω7c and 10-methyl C(16 : 0) and/or iso-C(17 : 1)ω9c. The polar lipids of strain SW150(T) comprised phosphatidylethanolamine, three unknown polar lipids and one unknown aminolipid. The major respiratory quinone was MK-6. The DNA G+C content of strain SW150(T) was 33.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina macrocephali JAMB N27(T) and Aquimarina muelleri KMM 6020(T) with 97.8 and 96.8% sequence similarities, respectively. The estimated DNA-DNA hybridization values were 21.00±2.33% between strain SW150(T) and A. macrocephali JAMB N27(T) and 20.60±2.32% between strain SW150(T) and Aquimarina megaterium XH134(T). On the basis of polyphasic analyses, strain SW150(T) represents a novel species of the genus Aquimarina, for which the name Aquimarina pacifica sp. nov. is proposed. The type strain is SW150(T) ( = JCM 18214(T) = CGMCC 1.12180(T)).