Can antioxidants protect against chemotherapy in a rat spermatogonial stem cell line?
Can antioxidants protect against chemotherapy in a rat spermatogonial stem cell line?
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DOI：
10.1530/raf-21-0042
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发表时间：
2021-12
影响因子：
2.1
通讯作者：
Spears N
中科院分区：
文献类型：
--
作者：
Allen CM;Lopes F;Mitchell RT;Spears N
接受化疗治疗癌症的男孩有健康睾丸组织受损的风险，这可能导致成年后不孕。因此，研究人员正在研究在癌症治疗期间保护睾丸的治疗方法。在这里，来自大鼠睾丸的细胞被培养4天，并在最后2天暴露于含有或不含抗氧化剂的化疗药物中。抗氧化剂可以通过灭活有毒化合物来减少细胞损伤。在这里，褪黑素或n-乙酰半胱氨酸等抗氧化剂与顺铂、阿霉素或长春新碱等化疗药物进行了对比试验。重复培养四次，在培养结束时测量细胞存活率。抗氧化剂对顺铂没有破坏作用，但对阿霉素没有保护作用。令人惊讶的是，n-乙酰半胱氨酸增强长春新碱引起的损伤。研究结果表明，使用抗氧化剂来保护睾丸，当与不同的化疗药物一起使用时，可能会产生有益或有害的效果：考虑到患者经常使用多种药物治疗，这一点很重要。
Boys administered chemotherapy to treat cancer are at risk of damage to their healthy testicular tissue, which can lead to infertility in adulthood. Researchers are therefore investigating treatments to protect the testis during cancer treatment. Here, cells originating from rat testicles were cultured for 4 days and exposed to chemotherapy drugs with or without antioxidants for the final 2 days. Antioxidants can reduce cellular damage by inactivating toxic compounds. Here, antioxidants such as melatonin or n-acetylcysteine were tested against chemotherapy agents cisplatin, doxorubicin, or vincristine. Cultures were repeated four times, with cell survival measured at the end of culture. The antioxidants were not damaging and partially protected against cisplatin, although not doxorubicin. Surprisingly, n-acetylcysteine enhanced vincristine-induced damage. The results suggest that using antioxidants to protect the testis could have either beneficial or harmful effects when given alongside different chemotherapy drugs: this is important, considering that patients are often treated with multiple drugs.