Nocardiosis in large yellow croaker, Larimichthys crocea (Richardson).
Nocardiosis in large yellow croaker, Larimichthys crocea (Richardson).
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DOI：
10.1111/j.1365-2761.2005.00637.x
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发表时间：
2005-06
影响因子：
2.5
通讯作者：
G.-L. Wang;S.-P. Yuan;S. Jin
中科院分区：
文献类型：
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作者：
G.-L. Wang;S.-P. Yuan;S. Jin
中国海水网箱养殖大黄鱼的流行病是由诺卡氏菌引起的。2003年8月至10月。累计死亡率为15%，病鱼为16个月龄，个体长度25~30 cm。心、脾、肾多发白色结节，直径0.1~0.2 cm。从Lowenstein-Jensen培养基和胰酶大豆琼脂中分离得到的细菌形态为珠状或细长的丝状杆状。实验感染表明，分离的细菌是引起死亡的病原菌。该菌的16S rRNA基因与模式菌株JCM 3360T(Z36925)的16S rRNA基因部分序列构成一个单系分支，序列相似性高达99.9%。根据形态、生理、生物学特性和系统发育分析，该病原菌被鉴定为串珠核菌。这是水产养殖中首次发现感染串珠丝虫的大黄鱼。
An epizootic in seawater-cage reared large yellow croaker, Larimichthys crocea, in China was caused by a Nocardia sp. from August to October 2003. The cumulative mortality rate was 15% and the diseased fish were 16 months old with individual length varying from 25 to 30 cm. Multiple, white nodules, 0.1-0.2 cm in diameter, were scattered on the heart, spleen and kidney. The morphology of isolated bacteria from Lowenstein-Jensen medium and tryptic soy agar was bead-like or long, slender, filamentous rods. Experimental infection indicated that the isolated bacterium was the pathogen responsible for the mortalities. A partial sequence of the 16S rRNA gene of the organism and the type strain of Nocardia seriolae JCM 3360T (Z36925) formed a monophyletic clade with a high sequence similarity of 99.9%. Based on the morphological, physiological, biological properties and the phylogenetic analysis, the pathogenic organism was identified as N. seriolae. This is the first report on N. seriolae-infected large yellow croaker in aquaculture.