Elevation of extracellular β-1,3-glucanase and chitinase activities in rose in response to treatment with acibenzolar-S-methyl and infection by D. rosae
Elevation of extracellular β-1,3-glucanase and chitinase activities in rose in response to treatment with acibenzolar-S-methyl and infection by D. rosae
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玫瑰细胞外 β-1,3-葡聚糖酶和几丁质酶活性的升高响应苯并-S-甲基处理和玫瑰果蝇感染
DOI：
10.1078/0176-1617-00300
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发表时间：
2001
影响因子：
4.3
通讯作者：
D. Leung
中科院分区：
文献类型：
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作者：
Y. Suo;D. Leung
摘要 在用苯并噻二唑（BTH，一种苯并噻二唑衍生物；商品名 Bion 50WG）处理或接种双果香后，在不同时间测定了体外玫瑰芽叶细胞间液中 β-1,3-葡聚糖酶和几丁质酶活性的变化。结果表明，BTH处理导致玫瑰叶细胞间隙中β-1,3-葡聚糖酶和几丁质酶的活性增强。在玫瑰果蝇感染的叶子中还发现细胞外β-1,3-葡聚糖酶和几丁质酶活性增加。然而，在之前用BTH处理的玫瑰花感染的叶子中，酶活性的增加发生得更快并且增强得更强烈，这表明增加的β-1,3-葡聚糖酶和几丁质酶可能在限制感染玫瑰花的玫瑰叶子上疾病症状的发展中发挥作用。使用特定染色技术在非变性 PAGE 上研究了玫瑰 β-1,3-葡聚糖酶和几丁质酶亚型的表达模式。在两种处理中观察到这些酶的相似诱导模式。 β-1,3-葡聚糖酶和几丁质酶活性增加主要是由于β-1,3-葡聚糖酶亚型G2和几丁质酶亚型C1和C2的表达增强。这些亚型可能是玫瑰对病原体攻击的防御反应的一部分。
Summary Changes in activities of β-1,3-glucanase and chitinase were determined in intercellular fluids of leaves of in vitro rose shoots at various times after treatment with acibenzolar-S-methyl (BTH, a benzothiadiazole derivative; trade name Bion 50WG) or inoculation with Diplocarpon rosae . The results indicate that BTH treatment led to enhanced activities of β-1,3-glucanase and chitinase in the intercellular spaces of rose leaves. An increase in extracellular β-1,3-glucanase and chitinase activities was also found in D. rosae -infected leaves. However, the increase in enzyme activities occurred much more rapidly and was more strongly enhanced in D. rosae -infected leaves that were previously treated with BTH, suggesting that the increased β-1,3-glucanase and chitinase may play a role in restricting the development of disease symptoms on the rose leaves infected with D. rosae . The expression patterns of rose β-1,3-glucanase and chitinase isoforms were investigated on native PAGE with specific staining techniques. A similar induction pattern of these enzymes was observed in both treatments. The increased β-1,3-glucanase and chitinase activities are mainly due to the enhanced expression of β-1,3-glucanase isoform G2 and chitinase isoforms C1 and C2. These isoforms are likely to be a part of rose defense responses to pathogen attack.
Osamu Tanaka：“一些商业蛋白质制剂对青萍 151 开花的影响”植物细胞生理学。
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