Cyclic stretch induces decorin expression via yes-associated protein in tenocytes: A possible mechanism for hyperplasia in masticatory muscle tendon-aponeurosis hyperplasia
Cyclic stretch induces decorin expression via yes-associated protein in tenocytes: A possible mechanism for hyperplasia in masticatory muscle tendon-aponeurosis hyperplasia
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DOI：
10.1016/j.ajoms.2018.12.012
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发表时间：
2019-05-01
影响因子：
0.4
通讯作者：
Yoda, Tetsuya
中科院分区：
文献类型：
--
作者：
Hayashi, Naoki;Sato, Tsuyoshi;Yoda, Tetsuya
目的：咀嚼肌腱腱膜增生症（MMTAH）是一种因双侧咀嚼肌腱增生和腱膜增生而导致张口受限的疾病。尽管尚不清楚 MMTAH 中肌腱的增生是否是由于循环拉伸所致，但我们假设循环拉伸是 MMTAH 受力的主要原因。在这项研究中，我们检测了 MMTAH 患者咀嚼肌腱中核心蛋白聚糖的表达。我们还检测了加载拉伸力的肌腱细胞的核心蛋白聚糖（Dcn）表达，以探讨拉伸力是否影响yes相关蛋白（YAP）信号传导以及Dcn表达是否受YAP调节。方法：对MMTAH患者的肌腱进行定量逆转录聚合酶链反应、免疫印迹分析和免疫组织化学分析。利用RNA干扰技术对TT-D6肌腱成纤维细胞进行机械加载实验。结果：我们发现MMTAH患者肌腱中Dcn表达增加。我们还表明，循环拉伸力增加了 TT-D6 肌腱成纤维细胞中核心蛋白聚糖的表达，并且在循环拉伸作用下的 TT-D6 肌腱成纤维细胞中，Dcn 表达通过 YAP 进行调节。结论：我们的结果首次证明，循环拉伸通过 YAP 在肌腱细胞中诱导核心蛋白聚糖表达，并且 Dcn 在 MMTAH 肌腱中上调，表明 MMTAH 肌腱受到循环拉伸条件的影响。
Objective: Masticatory muscle tendon-aponeurosis hyperplasia (MMTAH) is a disease which exhibits limiting mouth opening caused by hyperplasia of the tendon and aponeurosis of bilateral masticatory muscles. Although it is unclear whether hyperplasticity of tendons in MMTAH is due to cyclic stretch, we hypothesize that cyclic stretch is the main cause of force on MMTAH. In this study, we examined decorin expression in the masticatory muscle tendon of MMTAH patients. We also examined decorin (Dcn) expression of tenocytes on which stretch forces are loaded to explore whether stretch forces affect yes-associated protein (YAP) signaling and whether Dcn expression is regulated by YAP.Methods: Quantitative reverse-transcription polymerase chain reaction, immunoblot analysis and immunohistochemistry were performed in tendons of patients having MMTAH. Mechanical loading experiment for TT-D6 tendon fibroblastic cells using RNA interference technique was conducted.Results: We found that Dcn expression is increased in tendons of MMTAH patients. We also showed that cyclic stretch force increases decorin expression in TT-D6 tendon fibroblastic cells and that Dcn expression is regulated via YAP in TT-D6 tendon fibroblastic cells acted upon by cyclic stretch.Conclusions: Our results, for the first time, demonstrated that cyclic stretch induces decorin expression via YAP in tenocytes and Dcn is upregulated in tendons of MMTAH, suggesting that tendons of MMTAH were subjected to cyclic stretch conditions.