Bacterial cell curvature through mechanical control of cell growth
Bacterial cell curvature through mechanical control of cell growth
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DOI：
10.1038/emboj.2009.61
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发表时间：
2009-05-06
期刊：
影响因子：
11.4
通讯作者：
Jacobs-Wagner, Christine
中科院分区：
文献类型：
--
作者：
Cabeen, Matthew T.;Charbon, Godefroid;Jacobs-Wagner, Christine
细胞骨架是细胞形态发生的关键调节因子。Crescentin是一种细菌中间体，是新月柄杆菌弯曲形状所必需的，并定位于细胞内弯曲处。在这里，我们表明，crescentin形成一个单一的丝状结构，从细胞膜分离时，崩溃成一个螺旋，这表明它通常保持在一个拉伸配置。Crescentin引起围绕侧壁的圆周的伸长速率梯度，从而产生纵向单元长度差并因此产生曲率。当细胞在圆形微室中生长时，这种曲率可以仅通过物理力产生。在大肠杆菌中产生新月体素足以产生细胞弯曲。我们的数据认为，在该模型中，所承担的新月形结构的物理应变各向异性改变细胞壁插入的动力学，产生弯曲的增长。我们的研究表明，细菌可能使用细胞骨架进行机械控制生长，以改变形态。The EMBO Journal（2009）28，1208-1219. doi：10.1038/doj.2009.61; 2009年3月12日在线发布
The cytoskeleton is a key regulator of cell morphogenesis. Crescentin, a bacterial intermediate filament-like protein, is required for the curved shape of Caulobacter crescentus and localizes to the inner cell curvature. Here, we show that crescentin forms a single filamentous structure that collapses into a helix when detached from the cell membrane, suggesting that it is normally maintained in a stretched configuration. Crescentin causes an elongation rate gradient around the circumference of the sidewall, creating a longitudinal cell length differential and hence curvature. Such curvature can be produced by physical force alone when cells are grown in circular microchambers. Production of crescentin in Escherichia coli is sufficient to generate cell curvature. Our data argue for a model in which physical strain borne by the crescentin structure anisotropically alters the kinetics of cell wall insertion to produce curved growth. Our study suggests that bacteria may use the cytoskeleton for mechanical control of growth to alter morphology. The EMBO Journal (2009) 28, 1208-1219. doi: 10.1038/emboj.2009.61; Published online 12 March 2009